Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes

被引：49

作者：

Li, Olive T. W.

Barr, Ian

Leung, Connie Y. H.

Chen, Honglin

Guan, Yi

Peiris, J. S. Malik

Poon, Leo L. M. ^{[1
]}

机构：

[1] Univ Hong Kong, Queen Mary Hosp, Dept Microbiol, State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China

[2] WHO Collaborating Ctr Influenza, Melbourne, Vic, Australia

来源：

JOURNAL OF VIROLOGICAL METHODS | 2007年 / 142卷 / 1-2期

关键词：

influenza virus; polymerase genes; polymerase chain reaction; consensus primers;

D O I：

10.1016/j.jviromet.2007.01.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：218 / 222

页数：5

共 16 条

[1] Influenza type A in humans, mammals and birds: determinants of virus virulence, host-range and interspecies transmission [J].

Baigent, SJ ;

McCauley, JW .

BIOESSAYS, 2003, 25 (07) :657-671

[2] Amplification of the entire genome of influenza A virus H1N1 and H3N2 subtypes by reverse-transcription polymerase chain reaction [J].

Chan, Chi-Ho ;

Lin, Keh-Liang ;

Chan, You ;

Wang, Ya-Li ;

Chi, Yu-Tsai ;

Tu, Hsiao-Li ;

Shieh, Happy-K ;

Liu, Wu-Tse .

JOURNAL OF VIROLOGICAL METHODS, 2006, 136 (1-2) :38-43

[3] H5N1 virus outbreak in migratory waterfowl [J].

Chen, H ;

Smith, GJD ;

Zhang, SY ;

Qin, K ;

Wang, J ;

Li, KS ;

Webster, RG ;

Peiris, JSM ;

Guan, Y .

NATURE, 2005, 436 (7048) :191-192

[4] WebLogo: A sequence logo generator [J].

Crooks, GE ;

Hon, G ;

Chandonia, JM ;

Brenner, SE .

GENOME RESEARCH, 2004, 14 (06) :1188-1190

[5] Multiple introductions of H5N1 in Nigeria - Phylogenetic analysis reveals that this deadly virus first arrived in Africa from different sources [J].

Ducatez, M. F. ;

Olinger, C. M. ;

Owoade, A. A. ;

De Landtsheer, S. ;

Ammerlaan, W. ;

Niesters, H. G. M. ;

Osterhaus, A. D. M. E. ;

Fouchier, R. A. M. ;

Muller, C. P. .

NATURE, 2006, 442 (7098) :37-37

[6] Characterization of a novel influenza a virus hemagglutinin subtype (H16) obtained from black-headed gulls [J].

Fouchier, RAM ;

Munster, V ;

Wallensten, A ;

Bestebroer, TM ;

Herfst, S ;

Smith, D ;

Rimmelzwaan, GF ;

Olsen, B ;

Osterhaus, ADME .

JOURNAL OF VIROLOGY, 2005, 79 (05) :2814-2822

[7] Large-scale sequencing of human influenza reveals the dynamic nature of viral genome evolution [J].

Ghedin, E ;

Sengamalay, NA ;

Shumway, M ;

Zaborsky, J ;

Feldblyum, T ;

Subbu, V ;

Spiro, DJ ;

Sitz, J ;

Koo, H ;

Bolotov, P ;

Dernovoy, D ;

Tatusova, T ;

Bao, YM ;

St George, K ;

Taylor, J ;

Lipman, DJ ;

Fraser, CM ;

Taubenberger, JK ;

Salzberg, SL .

NATURE, 2005, 437 (7062) :1162-1166

[8] EVOLUTION OF INFLUENZA-A VIRUS PB2 GENES - IMPLICATIONS FOR EVOLUTION OF THE RIBONUCLEOPROTEIN COMPLEX AND ORIGIN OF HUMAN INFLUENZA A VIRUS [J].

GORMAN, OT ;

DONIS, RO ;

KAWAOKA, Y ;

WEBSTER, RG .

JOURNAL OF VIROLOGY, 1990, 64 (10) :4893-4902

[9] Universal primer set for the full-length amplification of all influenza A viruses [J].

Hoffmann, E ;

Stech, J ;

Guan, Y ;

Webster, RG ;

Perez, DR .

ARCHIVES OF VIROLOGY, 2001, 146 (12) :2275-2289

[10] Characterization of avian H3N3 and H1N1 influenza A viruses isolated from pigs in Canada [J].

Karasin, AI ;

West, K ;

Carman, S ;

Olsen, CW .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (09) :4349-4354

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