A tobacco flower-specific gene encodes a polyphenol oxidase

被引：27

作者：

Goldman, MHS

Seurinck, J

Marins, M

Goldman, GH

Mariani, C

机构：

[1] Univ Sao Paulo, FORP, Dept C Morfol, BR-14040904 Ribeirao Preto, SP, Brazil

[2] Plant Genet Syst NV, B-9000 Ghent, Belgium

[3] Univ Sao Paulo, FCFRP, Dept C Farmaceut, BR-14040934 Ribeirao Preto, SP, Brazil

[4] Catholic Univ Nijmegen, Dept Expt Bot, NL-6525 ED Nijmegen, Netherlands

来源：

PLANT MOLECULAR BIOLOGY | 1998年 / 36卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

flower-specific; Nicotiana tabacum; pistil-predominant expression; polyphenol oxidase; PPO; tobacco;

D O I：

10.1023/A:1005914918284

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Identification of pistil-expressed genes is an important step in understanding pistil development and function in plant reproduction. A tobacco stigma/style cDNA library was differentially screened and several cDNA clones were isolated. one of these tobacco genes, designated tobP1, is characterized here. TobP1 encodes a protein highly homologous to plant polyphenol oxidases. Northern blot analysis of total RNA extracted from different organs and probed with tobP1 cDNA identified a single transcript that is exclusively present in flower organs (petals, stamens, and predominantly in pistils). The tobP1 gene is co-ordinately regulated during development in pistils and stamens, and is not induced in mature leaves even under stress conditions. TobP1 belongs to a multigene family, as reported for PPO in other plant species.

引用

页码：479 / 485

页数：7