Immobilization of nucleic acids at solid surfaces: Effect of oligonucleotide length on layer assembly

被引:534
作者
Steel, AB
Levicky, RL
Herne, TM
Tarlov, MJ
机构
[1] Gene Log Inc, Gaithersburg, MD 20878 USA
[2] Columbia Univ, Dept Chem Engn & Appl Chem, New York, NY 10027 USA
[3] Natl Inst Stand & Technol, Proc Sensing Grp, Gaithersburg, MD 20899 USA
关键词
D O I
10.1016/S0006-3495(00)76351-X
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
This report investigates the effect of DNA length and the presence of an anchoring group on the assembly of presynthesized oligonucleotides at a gold surface. The work seeks to advance fundamental insight into issues that impact the structure and behavior of surface-immobilized DNA layers, as in, for instance, DNA microarray and biosensor devices. The present study contrasts immobilization of single-stranded DNA (ssDNA) containing a terminal, 5' hexanethiol anchoring group with that of unfunctionalized oligonucleotides for lengths from 8 to 48 bases. Qualitatively, the results indicate that the thiol anchoring group strongly enhances oligonucleotide immobilization, but that the enhancement is reduced for longer strand lengths. interestingly, examination of the probe coverage as a function of strand length suggests that adsorbed thiol-ssDNA oligonucleotides shorter than 24 bases tend to organize in end-tethered, highly extended configurations for which the long-term surface coverage is largely independent of oligonucleotide length. For strands longer than 24 bases, the surface coverage begins to decrease notably with probe length. The decrease is consistent with a less ordered arrangement of the DNA chains, presumably reflecting increasingly polymeric behavior.
引用
收藏
页码:975 / 981
页数:7
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