Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores

被引：102

作者：

Banerjee, Arijit ^{[1
]}

Mikhailova, Ellina ^{[1
]}

Cheley, Stephen ^{[1
]}

Gu, Li-Qun ^{[2
]}

Montoya, Michelle ^{[3
,4
]}

Nagaoka, Yasuo ^{[1
]}

Gouaux, Eric ^{[5
,6
]}

Bayley, Hagan ^{[1
]}

机构：

[1] Univ Oxford, Dept Chem, Oxford OX1 3TA, England

[2] Texas A&M Univ Syst, Hlth Sci Ctr, Dept Med Biochem & Genet, College Stn, TX 77843 USA

[3] Columbia Univ, Howard Hughes Med Inst, New York, NY 10032 USA

[4] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA

[5] Oregon Hlth & Sci Univ, Vollum Inst, Portland, OR 97239 USA

[6] Oregon Hlth & Sci Univ, Howard Hughes Med Inst, Portland, OR 97239 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2010年 / 107卷 / 18期

基金：

美国国家卫生研究院; 英国医学研究理事会;

关键词：

alpha-hemolysin; single molecule; stochastic sensing; structure; unnatural amino acid; CARBOHYDRATE-AROMATIC INTERACTIONS; ANTHRAX LETHAL TOXIN; SINGLE-MOLECULE; BETA-CYCLODEXTRIN; ALPHA-HEMOLYSIN; HYDROGEN-BONDS; FORMING PROTEIN; PI-INTERACTIONS; OUTER-MEMBRANE; ION CHANNELS;

D O I：

10.1073/pnas.0914229107

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Engineered protein pores have several potential applications in biotechnology: as sensor elements in stochastic detection and ultrarapid DNA sequencing, as nanoreactors to observe single-molecule chemistry, and in the construction of nano- and micro-devices. One important class of pores contains molecular adapters, which provide internal binding sites for small molecules. Mutants of the alpha-hemolysin (alpha HL) pore that bind the adapter beta-cyclodextrin (beta CD) similar to 10(4) times more tightly than the wild type have been obtained. We now use single-channel electrical recording, protein engineering including unnatural amino acid mutagenesis, and high-resolution x-ray crystallography to provide definitive structural information on these engineered protein nanopores in unparalleled detail.

引用

页码：8165 / 8170

页数：6

共 61 条

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