Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer

被引：33

作者：

Jefferies, J

Clarke, SC

Diggle, MA

Smith, A

Dowson, C

Mitchell, T

机构：

[1] N Glasgow Univ, Hosp NHS Tust, Dept Microbiol,Stobhill Hosp, Scottish Meningococcus & Pneumococcus Reference L, Glasgow G21 3UW, Lanark, Scotland

[2] Univ Glasgow, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland

[3] Glasgow Dent Hosp & Sch, Glasgow, Lanark, Scotland

[4] Univ Warwick, Dept Sci Biol, Coventry CV4 7AL, W Midlands, England

来源：

MOLECULAR BIOTECHNOLOGY | 2003年 / 24卷 / 03期

关键词：

multilocus sequence typing; DNA sequencing; polymerase chain reaction; automation;

D O I：

10.1385/MB:24:3:303

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Multilocus sequence typing (MLST) is used by the Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) as a routine method for the characterization of certain bacterial pathogens. The SMPRL recently started performing MLST on strains of Streptococcus pneumoniae, and here we describe a fully automated method for MLST using a 96-well-format liquid-handling robot and a 96-capillary automated DNA sequencer.

引用

页码：303 / 307

页数：5

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