Photoacoustic microscopy with 2-μm transverse resolution

被引：53

作者：

Ku, Geng ^{[1
]}

Maslov, Konstantin ^{[1
]}

Li, Li ^{[1
]}

Wang, Lihong V. ^{[1
]}

机构：

[1] Washington Univ, Dept Biomed Engn, Opt Imaging Lab, St Louis, MO 63130 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2010年 / 15卷 / 02期

关键词：

microscopy; photoacoustic microscopy; photoacoustic imaging; red blood cell; microcirculation; IN-VIVO; TOMOGRAPHY; SKIN;

D O I：

10.1117/1.3339912

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a new-generation optical-resolution confocal photoacoustic microscope, consisting of a 0.25 numerical aperture optical microscope objective and a 75-MHz center-frequency spherically focused ultrasonic transducer. Experiments verified that this microscope has a transverse resolution of 2 mu m, which is the highest to our knowledge among all photoacoustic imaging systems. In situ imaging of mouse ears shows the feasibility of resolving individual red blood cells in microvessels using the current system. (C) 2070 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3339912]

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