Profilin I colocalizes with speckles and Cajal bodies:: a possible role in pre-mRNA splicing

被引：78

作者：

Skare, P

Kreivi, JP

Bergstrom, Å

Karlsson, R ^{[1
]}

机构：

[1] Stockholm Univ, Wenner Gren Inst, Dept Cell Biol, SE-10691 Stockholm, Sweden

[2] Uppsala Univ, Dept Med Biochem & Microbiol, SE-75123 Uppsala, Sweden

来源：

EXPERIMENTAL CELL RESEARCH | 2003年 / 286卷 / 01期

关键词：

protilin; nuclear distribution; pre-mRNA splicing; Sm proteins; RNA-POLYMERASE-II; MESSENGER-RNA; COILED BODIES; MOLECULAR-CLONING; PROTEIN-PROFILIN; ACTIN DYNAMICS; NUCLEAR ACTIN; CELLS; GENE; ANTIBODIES;

D O I：

10.1016/S0014-4827(03)00102-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Profilin is one of the major components controlling actin polymerization. Here, profilin I was located in fibroblasts and HeLa cells by the use of two different sets of affinity-purified antibodies. Both antibody preparations labeled nuclei in a speckle-like pattern and displayed extensive colocalization with small nuclear ribonucleoprotein particle (snRNP)-core proteins and p80 coilin-containing Cajal bodies. Treatment with actinomycin D Led to Largely similar reorganizations of snRNPs and profilin, while profilin and Cajal bodies separated under these conditions. One of the profilin antibodies interfered with pre-mRNA splicing in vitro, further indicating a role for profilin during pre-mRNA processing. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：12 / 21

页数：10

共 59 条

[1]

Andersen JS, 2002, CURR BIOL, V12, P1, DOI 10.1016/S0960-9822(01)00650-9

[2] Regulation of nucleocytoplasmic trafficking by cell adhesion receptors and the cytoskeleton [J].