Vibrational microspectroscopy of food. Raman vs. FT-IR

被引:195
作者
Thygesen, LG [1 ]
Lokke, MM [1 ]
Micklander, E [1 ]
Engelsen, SB [1 ]
机构
[1] Royal Vet & Agr Univ, Dept Dairy & Food Sci, Ctr Adv Food Studies, DK-1958 Frederiksberg C, Denmark
关键词
D O I
10.1016/S0924-2244(02)00243-1
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
FT-IR and Raman spectroscopy are complementary techniques for the study of molecular vibrations and structure. The combination with a microscope results in an analytical method that allows spatially resolved investigation of the chemical composition of heterogeneous foods and food ingredients. The high spatial resolution makes it possible to study areas down to approximately 10x10 mum with FT-IR microspectroscopy and approximately 1 x 1 mum with Raman microspectroscopy. This presentation highlights the advantages and disadvantages of the two microspectroscopic techniques when applied to different heterogeneous food systems. FT-IR and Raman microspectroscopy were applied to a number of different problems related to food analysis: (1) in situ determination of starch and pectin in the potato cell, (2) in situ determination of the distribution of amygdalin in bitter almonds, (3) the composition of blisters found on the surface of bread, (4) the microstructure of high-lysine barley and (5) the composition of white spots in the shell of frozen shrimps. (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:50 / 57
页数:8
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