Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging

被引：153

作者：

Kou, Shan Shan ^{[1
,2
]}

Waller, Laura ^{[3
]}

Barbastathis, George ^{[4
,5
]}

Sheppard, Colin J. R. ^{[1
,2
,6
]}

机构：

[1] Natl Univ Singapore, Opt Bioimaging Lab, Div Bioengn, Singapore 117576, Singapore

[2] Natl Univ Singapore, Grad Sch Integrat Sci & Engn NGS, Singapore 117597, Singapore

[3] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA

[4] MIT, Dept Mech Engn, Cambridge, MA 02139 USA

[5] Singapore MIT Alliance Res & Technol SMART Ctr, Singapore 117597, Singapore

[6] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

来源：

OPTICS LETTERS | 2010年 / 35卷 / 03期

关键词：

RETRIEVAL; IMAGES;

D O I：

10.1364/OL.35.000447

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Differential interference contrast (DIC) microscopy is an inherently qualitative phase-imaging technique. What is obtained is an image with mixed phase-gradient and amplitude information rather than a true linear mapping of actual optical path length (OPL) differences. Here we investigate an approach that combines the transport-of-intensity equation (TIE) with DIC microscopy, thus improving direct visual observation. There is little hardware modification and the computation is noniterative. Numerically solving for the propagation of light in a series of through-focus DIC images allows linear phase information in a single slice to be completely determined and restored from DIC intensity values. (C) 2010 Optical Society of America

引用

页码：447 / 449

页数：3

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