An ethanol inducible gene switch for plants used to manipulate carbon metabolism

被引：198

作者：

Caddick, MX

Greenland, AJ

Jepson, I

Krause, KP

Qu, N

Riddell, KV

Salter, MG

Schuch, W

Sonnewald, U

Tomsett, AB ^{[1
]}

机构：

[1] Univ Liverpool, Sch Biol Sci, Donnan Labs, Liverpool L69 7ZD, Merseyside, England

[2] Zeneca Agrochem, Plant Biotechnol, Bracknell RG42 6ET, Berks, England

[3] IPK, D-06466 Gatersleben, Germany

来源：

NATURE BIOTECHNOLOGY | 1998年 / 16卷 / 02期

关键词：

agricultural biotechnology; inveterase; Aspergillus nidulans;

D O I：

10.1038/nbt0298-177

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Many transgenic plant studies use constitutive promoters to express transgenes. For certain genes, deleterious effects arise from constant expression in all tissues throughout development. We describe a chemically inducible plant gene expression system, with negligible background activity, that obviates this problem. We demonstrate its potential by showing inducible manipulation of carbon metabolism in transgenic plants. Upon rapid induction of yeast cytosolic invertase, a marked phenotype appears in developing leaves that is absent from leaves that developed before induction or after it has ceased.

引用

页码：177 / 180

页数：4

共 30 条

[21] IDENTIFICATION OF DNA-SEQUENCES REQUIRED FOR ACTIVITY OF THE CAULIFLOWER MOSAIC VIRUS-35S PROMOTER [J].