Evaluation of PCR primers from putative transcriptional regulator genes for identification of Staphylococcus aureus

被引：16

作者：

Liu, D ^{[1
]}

Lawrence, ML ^{[1
]}

Austin, FW ^{[1
]}

机构：

[1] Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Starkville, MS 39759 USA

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2005年 / 40卷 / 01期

关键词：

identification; PCR; Staphylococcus aureus; transcriptional regulator gene;

D O I：

10.1111/j.1472-765X.2004.01629.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: To examine if PCR primers derived from putative transcriptional regulator genes can be useful for Staphylococcus aureus identification. Methods and Results: Staphylococcus aureus gene sequences that encode transcriptional regulators were retrieved from GenBank and compared with other DNA sequences via BLAST searches. Two uniquely present, putative transcriptional regulator genes (i.e. Sa0836 and Sa0856) were selected as a consequence and PCR primers (Sa0836F/R and Sa0856F/R) were then designed from these genes for evaluation. A total of 84 bacterial strains/isolates including 23 Staph. aureus, 18 nonaureus Staphylococcus and 43 other common bacterial isolates were examined. The results indicated that PCR primers from Sa0836 and Sa0856 recognized genomic DNA from Staph. aureus only, but not from other non-aureus Staphylococcus or common bacteria. Conclusions: PCR detection of the putative transcriptional regulator genes Sa0836 and Sa0856 represents a useful means of identifying Staph. aureus from other bacteria. Significance and Impact ofthe Study: The existence of species-species transcriptional regulator genes may be a common phenomenon in bacteria. Besides their value as novel diagnostic markers, further investigation on the putative transcriptional regulator genes Sa0836 and Sa0856 and their related products may shed light on the molecular mechanisms of Staph. aureus adaptation and virulence.

引用

页码：69 / 73

页数：5

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