Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation

被引：200

作者：

Lee, M

Lenman, M

Banas, A

Bafor, M

Singh, S

Schweizer, M

Nilsson, R

Liljenberg, C

Dahlqvist, A

Gummeson, PO

Sjödahl, S

Green, A

Stymne, S ^{[1
]}

机构：

[1] Swedish Univ Agr Sci, Dept Plant Breeding Res, S-26831 Svalov, Sweden

[2] Svalov Welbull AB, S-26881 Svalov, Sweden

[3] CSIRO, Canberra, ACT 2601, Australia

[4] Inst Food Res, Norwich NR4 7UA, Norfolk, England

[5] Univ Gothenburg, Dept Plant Physiol, S-41319 Gothenburg, Sweden

来源：

SCIENCE | 1998年 / 280卷 / 5365期

关键词：

D O I：

10.1126/science.280.5365.915

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Acetylenic bonds are present in more than 600 naturally occurring compounds. Plant enzymes that catalyze the formation of the Delta 12 acetylenic bond in 9-octadecen-12-ynoic acid and the Delta 12 epoxy group in 12,13-epoxy-9-octadecenoic acid were characterized, and two genes, similar in sequence, were cloned. When these complementary DNAs were expressed in Arabidopsis thaliana, the content of acetylenic or epoxidated fatty acids in the seeds increased from 0 to 25 or 15 percent, respectively. Both enzymes have characteristics similar to the membrane proteins containing non-heme iron that have histidine-rich motifs.

引用

页码：915 / 918

页数：4

共 23 条

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