Comparative fitness assessment of Anopheles stephensi transgenic lines receptive to site-specific integration

被引：36

作者：

Amenya, D. A. ^{[2
,3
]}

Bonizzoni, M. ^{[2
,3
]}

Isaacs, A. T. ^{[1
]}

Jasinskiene, N. ^{[3
]}

Chen, H. ^{[2
]}

Marinotti, O. ^{[3
]}

Yan, G. ^{[2
]}

James, A. A. ^{[1
,3
]}

机构：

[1] Univ Calif Irvine, Dept Microbiol & Mol Genet, Irvine, CA 92697 USA

[2] Univ Calif Irvine, Program Publ Hlth, Irvine, CA 92697 USA

[3] Univ Calif Irvine, Dept Mol Biol & Biochem, Irvine, CA 92697 USA

来源：

INSECT MOLECULAR BIOLOGY | 2010年 / 19卷 / 02期

关键词：

life-table analyses; site-specific recombination; hybridizations in situ; GERMLINE TRANSFORMATION; GENETIC MANIPULATION; GAMBIAE; VECTOR; CELLS;

D O I：

10.1111/j.1365-2583.2009.00986.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetically modified mosquitoes that are unable to transmit pathogens offer opportunities for controlling vector-borne diseases such as malaria and dengue. Site-specific gene recombination technologies are advantageous in the development of these insects because antipathogen effector genes can be inserted at integration sites in the genome that cause the least alteration in mosquito fitness. Here we describe Anopheles stephensi transgenic lines containing phi C31 attP 'docking' sites linked to a fluorescent marker gene. Chromosomal insertion sites were determined and life-table parameters were assessed for transgenic mosquitoes of each line. No significant differences in fitness between the transgenic and nontransgenic mosquitoes were detected in this study. These transgenic lines are suitable for future site-specific integrations of antiparasite transgenes into the attP sites.

引用

页码：263 / 269

页数：7

共 19 条

[11] Fitness consequences of Anopheles gambiae population hybridization -: art. no. 44 [J].

Menge, DM ;

Guda, T ;

Zhong, DB ;

Pai, A ;

Zhou, GF ;

Beier, JC ;

Gouagna, L ;

Yan, GY .

MALARIA JOURNAL, 2005, 4 (1)

[12] High efficiency site-specific genetic engineering of the mosquito genome [J].

Nimmo, DD ;

Alphey, L ;

Meredith, JM ;

Eggleston, P .

INSECT MOLECULAR BIOLOGY, 2006, 15 (02) :129-136

[13]

SAMBROOK J, 1989, LAB MANUAL, P31

[14]

Sharakhova MV, 2006, J MED ENTOMOL, V43, P861, DOI 10.1603/0022-2585(2006)43[861:ASCPFT]2.0.CO

[15]

[16]

Sokal R.R, 1995, BIOMETRY PRINCIPLES

[17] Molecular Genetic Manipulation of Vector Mosquitoes [J].

Terenius, Olle ;

Marinotti, Osvaldo ;

Sieglaff, Douglas ;

James, Anthony A. .

CELL HOST & MICROBE, 2008, 4 (05) :417-423

[18]

Terenius Olle, 2007, J Vis Exp, P216, DOI 10.3791/216

[19]

TRASK BJ, 1991, METHOD CELL BIOL, V35, P3

← 1 2 →