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Biochemical and molecular characterization of Leishmania parasites isolated from an endemic focus in eastern Sudan

被引：26
作者：
Oskam, L
Pratlong, F
Zijlstra, EE
Kroon, CCM
Dedet, JP
Kager, PA
Schonian, G
Ghalib, HW
El-Hassan, AM
Meredith, SEO
机构：
[1] Royal Trop Inst, Dept Biomed Res, NL-1105 AZ Amsterdam, Netherlands
[2] Fac Med Montpellier, Lab Ecol Med & Pathol Parasitaire, Montpellier, France
[3] Univ Amsterdam, Acad Med Ctr, Dept Infect Dis Trop Med & AIDS, NL-1105 AZ Amsterdam, Netherlands
[4] Univ Khartoum, Inst Endem Dis, Khartoum, Sudan
[5] Humboldt Univ, Klinikum Charite, Inst Mikrobiol & Hyg, Berlin, Germany
来源：
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1998年 / 92卷 / 01期
关键词：
leishmaniasis; visceral leishmaniasis; post kala-azar dermal leishmaniasis; Leishmania donovani; Leishmania infantum; Leishmania archibaldi; characterization; zymodemes; Southern blotting; polymerase chain reaction; Sudan;
D O I：
10.1016/S0035-9203(98)90982-8
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Twelve Leishmania isolates from visceral leishmaniasis patients in eastern Sudan were characterized using isoenzyme analysis, Southern blotting and polymerase chain reaction (PCR) 'fingerprinting'. Isoenzyme analysis revealed the presence of 3 zymodemes: MON-18, MON-30 and MON-82, corresponding to Leishmania donovani sensu stricto, L. infantum and L. archibaldi (still of uncertain taxonomic status), respectively. Southern blotting and PCR 'fingerprinting' revealed identical patterns for all 3 zymodemes, which were indistinguishable from those of L. donovani s.s.
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收藏
页码：120 / 122
页数：3
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