Insulin degrading enzyme is expressed in the human cerebrovascular endothelium and in cultured human cerebrovascular endothelial cells

被引：25

作者：

Gao, WW

Eisenhauer, PB

Conn, K

Lynch, JA

Wells, JM

Ullman, MD

McKee, A

Thatte, HS

Fine, RE ^{[1
]}

机构：

[1] ENR VA Med Ctr, Bedford, MA 01730 USA

[2] Boston Univ, Sch Med, Dept Neurol, Boston, MA 02118 USA

[3] Univ Massachusetts, Sch Med, Shriver Ctr, Waltham, MA 02452 USA

[4] Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 USA

[5] Boston Univ, Sch Med, Dept Pathol, Boston, MA 02118 USA

[6] Harvard Univ, Sch Med, Brockton W Roxbury Vet Affairs Med Ctr, Boston, MA 01255 USA

[7] Harvard Univ, Sch Med, Dept Surg, Boston, MA 01225 USA

来源：

NEUROSCIENCE LETTERS | 2004年 / 371卷 / 01期

关键词：

insulin degrading enzyme (insulysin); cerebral endothelium; A beta catabolism;

D O I：

10.1016/j.neulet.2004.07.034

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Insulin degrading enzyme (IDE) is found in the cytosol, peroxisomes and plasma membrane of many cells. Although it preferentially cleaves insulin it can also cleave many other small proteins with diverse sequences including the monomeric form of the amyloid beta peptide (Abeta). In the brain, IDE has been reported to be expressed predominantly in neurons. In this study, IDE expression was detected in cultured human cerebrovascular endothelial cells. Using laser capture microdissection followed by PCR analysis, it was found that IDE mRNA is expressed in human brain blood vessels. Using immunofluorescence and multiphoton microscopy IDE was localized to the endothelium of the cerebrovascular blood vessels in human. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

引用

收藏

页码：6 / 11

页数：6

相关论文

共 39 条

[1] Substantial linkage disequilibrium across the insulin-degrading enzyme locus but no association with late-onset Alzheimer's disease [J].

Abraham, R ;

Myers, A ;

Wavrant-DeVrieze, F ;

Hamshere, ML ;

Thomas, HV ;

Marshall, H ;

Compton, D ;

Spurlock, G ;

Turic, D ;

Hoogendoorn, B ;

Kwon, KM ;

Petersen, RC ;

Tangalos, E ;

Norton, J ;

Morris, JC ;

Bullock, R ;

Liolitsa, S ;

Lovestone, S ;

Hardy, J ;

Goate, A ;

O'Donovan, M ;

Williams, J ;

Owen, MJ ;

Jones, L .

HUMAN GENETICS, 2001, 109 (06) :646-652

[2] Confirmation of association between D10S583 and Alzheimer's disease in a case-control sample [J].

Ait-Ghezala, G ;

Abdullah, L ;

Crescentini, R ;

Crawford, F ;

Town, T ;

Singh, S ;

Richards, D ;

Duara, R ;

Mullan, M .

NEUROSCIENCE LETTERS, 2002, 325 (02) :87-90

[3]

Authier F, 1996, CLIN INVEST MED, V19, P149

[4] Isolating vessels from the mouse brain for gene expression analysis using laser capture microdissection [J].

Ball, HJ ;

McParland, B ;

Driussi, C ;

Hunt, NH .

BRAIN RESEARCH PROTOCOLS, 2002, 9 (03) :206-213

[5] Insulin-degrading enzyme in the Alzheimer's disease brain:: prominent localization in neurons and senile plaques [J].

Bernstein, HG ;

Ansorge, S ;

Riederer, P ;

Reiser, M ;

Frölich, L ;

Bogerts, B .

NEUROSCIENCE LETTERS, 1999, 263 (2-3) :161-164

[6] Evidence for genetic linkage of Alzheimer's disease to chromosome 10q [J].

Bertram, L ;

Blacker, D ;

Mullin, K ;

Keeney, D ;

Jones, J ;

Basu, S ;

Yhu, S ;

McInnis, MG ;

Go, RCP ;

Vekrellis, K ;

Selkoe, DJ ;

Saunders, AJ ;

Tanzi, RE .

SCIENCE, 2000, 290 (5500) :2302-+

[7] Sequence variation in the proximity of IDE may impact age at onset of both Parkinson disease and Alzheimer disease [J].

Blomqvist, MEL ;

Silburn, PA ;

Buchanan, DD ;

Andreasen, N ;

Blennow, K ;

Pedersen, NL ;

Brookes, AJ ;

Mellick, GD ;

Prince, JA .

NEUROGENETICS, 2004, 5 (02) :115-119

[8] CELLULAR-DISTRIBUTION OF INSULIN-DEGRADING ENZYME GENE-EXPRESSION - COMPARISON WITH INSULIN AND INSULIN-LIKE GROWTH-FACTOR RECEPTORS [J].

BONDY, CA ;

ZHOU, J ;

CHIN, E ;

REINHARDT, RR ;

DING, L ;

ROTH, RA .

JOURNAL OF CLINICAL INVESTIGATION, 1994, 93 (03) :966-973

[9] Polymorphisms of insulin degrading enzyme gene are not associated with Alzheimer's disease [J].

Boussaha, M ;

Hannequin, D ;

Verpillat, P ;

Brice, A ;

Frebourg, T ;

Campion, D .

NEUROSCIENCE LETTERS, 2002, 329 (01) :121-123

[10] Insulin-degrading enzyme does not require peroxisomal localization for insulin degradation [J].

Chesneau, V ;

Perlman, RK ;

Li, WL ;

Keller, GA ;

Rosner, MR .

ENDOCRINOLOGY, 1997, 138 (08) :3444-3451

← 1 2 3 4 →