Protective effect of pilose antler peptide on cerebral ischemia/reperfusion (I/R) injury through Nrf-2/OH-1/NF-κB pathway

被引：31

作者：

Bai, Luning ^{[1
,2
]}

Shi, Wei ^{[1
]}

Liu, Jiping ^{[3
]}

Zhao, Xiaoping ^{[2
]}

Zhang, Yi ^{[2
]}

Zhou, Zhengguo ^{[2
]}

Hou, Wen ^{[2
]}

Chang, Tao ^{[2
]}

机构：

[1] Xian Jiao Tong Univ Xian, Med Coll, Xian 71000, Shaanxi, Peoples R China

[2] Shaanxi Univ Chinese Med, Affiliated Hosp, Xianyang 712046, Peoples R China

[3] Shaanxi Univ Chinese Med, Dept Pharmacol, Xianyang 712046, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2017年 / 102卷

关键词：

Pilose antler peptide; Middle cerebral artery occlusion; Nrf-2/HO-1/NF-kappa B; ISCHEMIA-REPERFUSION INJURY; ACUTE LUNG INJURY; ANTIISCHEMIC STROKE AGENT; NF-KAPPA-B; CANCER-THERAPY; BETULINIC ACID; INFLAMMATION; SALIDROSIDE; CHRYSOPHANOL; MECHANISMS;

D O I：

10.1016/j.ijbiomac.2017.04.091

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The purpose of the current study was to detect the effect of pilose antler peptide (PAP) on cerebral ischemia and explore its potential mechanism. Middle cerebral artery occlusion (MCAO) was performed to investigate the effects of PAP on cerebral ischemia. The rats were randomly divided into five groups: sham group, model(I/R) group, I/R+ Clopidogrel group, I/R+ PAP (50 mg/kg) group, I/R+ PAP (100 mg/kg) group. The results showed that PAP significantly reduced brain infarct size, inhibited cerebral edema, decreased th eneurological deficits. Treatment with PA reduced the contents of malondialdehyde(MDA) and restore the activity of superoxide dismutase(SOD) in serum and brains. Lig also could effectively decrease the levels of interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in serum and brain tissues of the MCAO rats. Western blot results demonstrated that PAP up-regulated the expressions of Nrf-2 and HO-1, also blocked the phosphorylations of I kappa B kinases(IKK)alpha, IKK beta, inhibitor of NF-kappa B(I kappa B)alpha, nuclear factor kappa B(NF-kappa B) p65 in I/R-induced brains. Our findings suggested that PAP effectively attenuated the I/R-induced cerebral ischemia through Nrf-2/HO-1/NF-kappa B pathway. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：741 / 748

页数：8

共 28 条

[1]

RETRACTED: Secretory phospholipase A2 IIA is up-regulated by TNF-α and IL-1α/β after transient focal cerebral ischemia in rat (Retracted article. See vol. 1507, pg. 154, 2013) [J].