Electrochemical optical waveguide lightmode spectroscopy (EC-OWLS):: A pilot study using evanescent-field optical sensing under voltage control to monitor polycationic polymer adsorption onto indium tin oxide (ITO)-coated waveguide chips

被引:60
作者
Bearinger, JP
Vörös, J
Hubbell, JA
Textor, M
机构
[1] Swiss Fed Inst Technol, Oberflachentech Lab Surface Sci & Technol, CH-8952 Schlieren, Switzerland
[2] Swiss Fed Inst Technol, Swiss Fed Inst Technol, Inst Biomed Engn, Zurich, Switzerland
关键词
optical evanescent field sensor; indium tin oxide (ITO); electrical potential; adsorption; polyionic polymers; biosensor;
D O I
10.1002/bit.10591
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A new technique has been developed that combines evanescent-field optical sensing with electrochemical control of surface adsorption processes. This new technique, termed "electrochemical optical waveguide lightmode spectroscopy" (EC-OWLS), proved efficient in monitoring molecular surface adsorption and layer thickness changes of an adsorbed polymer layer examined in situ as a function of potential applied to a waveguide in a pilot study. For optical sensing, a layer of indium tin oxide (ITO) served as both a high-refractive-index waveguide and a conductive electrode. In addition, an electrochemical flow-through fluid cell was provided, which incorporated working, reference, and counter electrodes, and was compatible with the constraints of optical sensing. POIY(L-lysine)-grafted-poly(ethylene glycol) (PLL-g-PEG) served as a model, polycation adsorbate. Adsorption of PLL-g-PEG from aqueous buffer solution increased from 125 to 475 ng/cm(2) along a sigmoidal path. as a function of increasing potential between 0 and 1.5 V versus the Ag reference electrode. Upon buffer rinse, adsorption was partially reversible when a potential of greater than or equal to0.93 V was maintained on the ITO waveguide. However, reducing the applied potential back to 0 V before rinsing resulted in irreversible polymer adsorption. PLL-g-PEG modified with biotin demonstrated similar adsorption characteristics, but subsequent streptavidin binding was independent of biotin concentration. Applying positive potentials resulted in increased adsorbed mass, presumably due to polymer chain extension and reorganization in the molecular adlayer. (C) 2003 Wiley Periodicals, Inc.
引用
收藏
页码:465 / 473
页数:9
相关论文
共 46 条
[21]   Poly(L-lysine)-g-poly(ethylene glycol) layers on metal oxide surfaces:: Attachment mechanism and effects of polymer architecture on resistance to protein adsorption [J].
Kenausis, GL ;
Vörös, J ;
Elbert, DL ;
Huang, NP ;
Hofer, R ;
Ruiz-Taylor, L ;
Textor, M ;
Hubbell, JA ;
Spencer, ND .
JOURNAL OF PHYSICAL CHEMISTRY B, 2000, 104 (14) :3298-3309
[22]   Plasma protein adsorption on titanium: comparative in situ studies using optical waveguide lightmode spectroscopy and ellipsometry [J].
Kurrat, R ;
Walivaara, B ;
Marti, A ;
Textor, M ;
Tengvall, P ;
Ramsden, JJ ;
Spencer, ND .
COLLOIDS AND SURFACES B-BIOINTERFACES, 1998, 11 (04) :187-201
[23]   Instrumental improvements in optical waveguide light mode spectroscopy for the study of biomolecule adsorption [J].
Kurrat, R ;
Textor, M ;
Ramsden, JJ ;
Boni, P ;
Spencer, ND .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1997, 68 (05) :2172-2176
[24]   Protein adsorption onto auto-assembled polyelectrolyte films [J].
Ladam, G ;
Schaaf, P ;
Cuisinier, FJG ;
Decher, G ;
Voegel, JC .
LANGMUIR, 2001, 17 (03) :878-882
[25]   ELECTROCHEMICALLY-CONTROLLED WAVE-GUIDE-COUPLED SURFACE-PLASMON SENSING [J].
LAVERS, CR ;
HARRIS, RD ;
HAO, S ;
WILKINSON, JS ;
ODWYER, K ;
BRUST, M ;
SCHIFFRIN, DJ .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1995, 387 (1-2) :11-22
[26]   BLOOD COMPATIBILITY OF POLYETHYLENE OXIDE SURFACES [J].
LEE, JH ;
LEE, HB ;
ANDRADE, JD .
PROGRESS IN POLYMER SCIENCE, 1995, 20 (06) :1043-1079
[27]  
LIRON Z, 2002, IN PRESS BIOSENS BIO
[28]   SENSITIVITY OF INTEGRATED OPTICAL GRATING AND PRISM COUPLERS AS (BIO)CHEMICAL SENSORS [J].
LUKOSZ, W ;
TIEFENTHALER, K .
SENSORS AND ACTUATORS, 1988, 15 (03) :273-284
[30]   DISCRIMINATION BETWEEN PHYSICAL AND CHEMICAL ADSORPTION OF IONS ON OXIDES [J].
LYKLEMA, J .
COLLOIDS AND SURFACES, 1989, 37 :197-204