PGC-1 and PERC, coactivators of the estrogen receptor-related receptor γ

被引：62

作者：

Hentschke, M ^{[1
]}

Süsens, U ^{[1
]}

Borgmeyer, U ^{[1
]}

机构：

[1] Univ Hamburg, Inst Entwicklungsneurobiol, Zentrum Mol Neurobiol, D-20246 Hamburg, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 299卷 / 05期

关键词：

transcription factor; ERR3; NR3B3; nuclear localization; EGFP; transactivation; pull down;

D O I：

10.1016/S0006-291X(02)02753-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mouse nuclear receptor ERRgamma (estrogen receptor-related receptor 7) is highly expressed in heart, skeletal muscle, kidney, and brain, as well as in the developing nervous system. We found that the expression of the coactivators PGC-1 (PGC-1alpha) and PERC (PGC-1beta) in mammalian cells augmented potently the transcriptional activation by ERRgamma. The constitutive activation function 2 (AF-2) of the orphan receptor was important for the synergistic enhancement. Functional receptor truncation analysis revealed an additional amino-terminal activation function, specific for the ERRgamma2 isoform and PGC-1. In vitro experiments showed a direct interaction of ERRgamma with both coactivators. Our findings suggest distinct regulatory functions for PGG-1 and PERC as tissue-specific coactivators for ERRgamma. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：872 / 879

页数：8

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