Rapid high-performance liquid chromatographic method of determining malondialdehyde for evaluation of rancidity in edible oils

被引：39

作者：

Tsaknis, J

Lalas, S

Hole, M

Smith, G

Tychopoulos, V

机构：

[1] Technol Educ Inst Athens, Dept Food Technol, Athens 12210, Greece

[2] Univ Humberside, Sch Appl Sci & Technol, Grimsby DN34 5AA, England

来源：

ANALYST | 1998年 / 123卷 / 02期

关键词：

malondialdehyde; high-performance liquid chromatographic analysis; thiobarbituric acid analysis; vegetable oil; oxidation;

D O I：

10.1039/a706812c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple HPLC method for the determination of malondialdehyde (MDA) in oxidised vegetable oils was developed and the results were compared with those given by a spectrophotometric method, Vegetable oil was steam-distilled in a Kjeldahl distillation apparatus and the MDA was determined in the aqueous distillates by HPLC, using a mu-Bondapak C-18 column, with a mobile phase of 1% acetic acid-acetonitrile (85 + 15, v/v), A total time of 2 min was necessary to assay each distillate and only MDA was detected, MDA can be determined at a level of 1.5 x 10(-9) mol l(-1).

引用

页码：325 / 327

页数：3

共 13 条

[1] THIOBARBITURIC ACID REACTION AND AUTOXIDATIONS OF POLYUNSATURATED FATTY ACID METHYL ESTERS [J].