Chromosomal 20q gain in the DNA diploid component of aneuploid colorectal carcinomas

被引:14
作者
De Angelis, Paula M.
Stokke, Trond
Beigi, Marzieh
Flatberg, Goril
Enger, Marianne
Haug, Kristiane
Aass, Hans Christian D.
Schjolberg, Aasa
Andresen, Per Arne
Ariansen, Sarah
Bo, Anne Signe
Mjaland, Odd
Clausen, Ole Petter
机构
[1] Univ Oslo, Rikshosp Radiumhosp Med Ctr, Pathol Clin, N-0027 Oslo, Norway
[2] Rikshosp Radiumhosp Med Ctr, Inst Canc Res, N-0027 Oslo, Norway
[3] Rikshosp Radiumhosp Med Ctr, Dept Mol Pathol, N-0027 Oslo, Norway
[4] Sorlandet Hosp, Dept Gastrosurg, N-4604 Kristiansand, Norway
关键词
DNA aneuploidy; DNA diploidy; flow-sorted colorectal tumors; CGH; chromosomal aberrations; FISH; 20q13; gain;
D O I
10.1002/ijc.22537
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The order of appearance of different genetic aberrations during the shift from diploidy/near-diploidy to aneuploidy in colorectal cancers is not yet clear. We studied genetic alterations in flow cytometrically-sorted DNA diploid and corresponding aneuploid epithelial cell populations from each of 20 colorectal tumors using comparative genomic hybridization, FISH, and PCR. Analysis of the 19 cases in which aberrations were found in the flow-sorted diploid population indicated that large-scale aneuploidization in colorectal cancer was preceded by amplification of oncogene(s) localized to chromosome 20q13.2 and by KRAS mutations, but not by TP53 deletions or losses of large chromosomal regions such as 4q, 8p and 18q. (c) 2007 Wiley-Liss, Inc.
引用
收藏
页码:2734 / 2738
页数:5
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