The interplay between microRNAs and the neurotrophin receptor tropomyosin-related kinase C controls proliferation of human neuroblastoma cells

被引:114
作者
Laneve, Pietro
Di Marcotullio, Lucia
Gioia, Ubaldo
Fiori, Micol E.
Ferretti, Elisabetta
Gulino, Alberto
Bozzoni, Irene
Caffarelli, Elisa
机构
[1] Univ Roma La Sapienza, CNR, Inst Mol Biol & Pathol, I-00185 Rome, Italy
[2] Univ Roma La Sapienza, Inst Pasteur Cenci Bolognetti, Dept Genet & Mol Biol, I-00185 Rome, Italy
[3] Univ Roma La Sapienza, Dept Expt Med, I-00185 Rome, Italy
关键词
miR-9; miR-125a; miR125b; tyrosine kinase receptor;
D O I
10.1073/pnas.0700071104
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
MicroRNAs (miRNAs) are tiny noncoding RNAs whose function as modulators of gene expression is crucial for the proper control of cell growth and differentiation. Although the profile of miRNA expression has been defined for many different cellular systems, the elucidation of the regulatory networks in which they are involved is only just emerging. In this work, we identify a crucial role for three neuronal miRNAs (9, 125a, and 125b) in controlling human neuroblastoma cell proliferation. We show that these molecules act in an additive manner by repressing a common target, the truncated isoform of the neurotrophin receptor tropomyosin-related kinase C, and we demonstrate that the down-regulation of this isoform is critical for regulating neuroblastoma cell growth. Consistently with their function, these miRNAs were found to be down-modulated in primary neuroblastoma tumors.
引用
收藏
页码:7957 / 7962
页数:6
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