Dual metabolomics: A novel approach to understanding plant-pathogen interactions

被引：58

作者：

Allwood, J. William ^{[1
,2
]}

Clarke, Andrew ^{[1
]}

Goodacre, Royston ^{[2
,3
]}

Mur, Luis A. J. ^{[1
]}

机构：

[1] Aberystwyth Univ, IBERS, Aberystwyth SY23 3DA, Dyfed, Wales

[2] Univ Manchester, Sch Chem, Manchester Interdisciplinary Bioctr, Manchester M1 7DN, Lancs, England

[3] Univ Manchester, Manchester Ctr Integrat Syst Biol, Manchester Interdisciplinary Bioctr, Manchester M1 7DN, Lancs, England

来源：

PHYTOCHEMISTRY | 2010年 / 71卷 / 5-6期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

Dual metabolomics; Arabidopsis thaliana; Pseudomonas syringae pv. tomato; Fourier transform infrared (FT-IR) spectroscopy; PROGRAMMED CELL-DEATH; SYRINGAE PV. TOMATO; PSEUDOMONAS-SYRINGAE; EFFECTOR PROTEINS; NITRIC-OXIDE; RESISTANCE; EXPRESSION; TRANSCRIPTOME; RESPONSES; SIGNAL;

D O I：

10.1016/j.phytochem.2010.01.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

One of the most well-characterised plant pathogenic interactions involves Arabidopsis thaliana and the bacteria Pseudomonas syringae pathovar tomato (Pst). The standard Pst inoculation procedure involves infiltration of large populations of bacteria into plant leaves which means that metabolite changes cannot be readily assigned to the host or pathogen. A plant cell-pathogen co-culture based approach has been developed where the plant and pathogen cells are separated after 12 h of co-culture via differential filtering and centrifugation. Fourier transform infrared (FT-IR) spectroscopy was employed to assess the intracellular metabolomes (metabolic fingerprints) of both host and pathogen and their extruded (extracellular) metabolites (metabolic footprints) under conditions relevant to disease and resistance. We propose that this system will enable the metabolomic profiling of the separated host and pathogen (i.e. 'dual metabolomics') and will facilitate the modelling of reciprocal responses. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：590 / 597

页数：8

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