A functional assay in Escherichia coli to detect non-assisted interaction between galactose repressor dimers

被引:7
作者
Perez, N [1 ]
Rehault, M [1 ]
Amouyal, M [1 ]
机构
[1] Inst Gustave Roussy PR2, Equipe Interact Distance, CNRS, UMR 8532, F-94805 Villejuif, France
关键词
D O I
10.1093/nar/28.18.3600
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Among the Escherichia coli operons repressed from multiple sites on DNA, the galactose operon is unique: its repression requires an auxiliary protein, HU, to assist cooperative repressor binding to two distant DNA sites. Here we show that GalR can still mediate repression from distant sites in an artificial and simplified regulatory region which totally disturbs the organisation of the natural interactions. This simple and unexpected cooperation of a protein incapable Of self-association in solution might be involved in regulation of the gal operon. Furthermore, the assay may be generalised to detection of rather weak cooperative interactions between DNA-bound proteins.
引用
收藏
页码:3600 / 3604
页数:5
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