Multiple protein-binding sites in the TAS-region of human and rat mitochondrial DNA

被引:41
作者
Roberti, M
Musicco, C
Polosa, PL
Milella, F
Gadaleta, MN
Cantatore, P
机构
[1] Univ Bari, Dipartmento Biochim & Biol Mol, I-70126 Bari, Italy
[2] CNR, Ctr Studi Mitocondri & Metab Energet, I-70126 Bari, Italy
关键词
D O I
10.1006/bbrc.1997.8052
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To study the molecular mechanisms responsible for the regulation of mitochondrial DNA copy number, in vivo and in organello dimethyl sulfate footprinting experiments in human fibroblasts and rat liver mitochondria were carried out. By this approach we identified in both species two specific protein binding sites in the 3' region of the displacement loop of mitochondrial DNA. One site contains the TAS-D element of human and rat mitochondrial DNA; the other covers TAS-C and TAS-B in human, whereas in rat it comprises part of TAS-B. We suggest that the protected sequences might be the site of action of protein factors involved in the premature termination of mitochondrial DNA heavy-strand synthesis. (C) 1998 Academic Press.
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页码:36 / 40
页数:5
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