High-performance liquid chromatography electrospray mass spectrometric analysis of pungent constituents of ginger

被引:95
作者
He, XG [1 ]
Bernart, MW [1 ]
Lian, LZ [1 ]
Lin, LZ [1 ]
机构
[1] E Earth Herb Inc, Res Lab Nat Prod Chem, Eugene, OR 97402 USA
关键词
Zingiber officinale; ginger; gingerol; shogaol;
D O I
10.1016/S0021-9673(97)01013-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A gradient elution reversed-phase HPLC separation of ginger extract has been developed, which gives better separation than previous isocratic separations. HPLC-UV-electrospray MS has been successfully utilized to identify the individual pungent constituents in the chromatogram of ginger extract. Seven compounds were positively identified as the major pungent constituents of ginger based on their UV spectra, [M+H](+), [M+Na](+) and characteristic sodiated dimer [2M+Na](+) ions, and comparison to data for the purified standards: [6]-gingerol and [6]-shogaol. The pungent compounds were assigned as [6]-gingerol, [8]-gingerol, [10]-gingerol, [6]-shogaol, [8]-shogaol, [IO]-shogaol and [6]-gingediol. Another eight minor compounds were tentatively identified as gingerol analogues. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:327 / 334
页数:8
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