Microenvironments of pH in biofilms grown on dissolving silicate surfaces

被引:68
作者
Liermann, LJ [1 ]
Barnes, AS
Kalinowski, BE
Zhou, XY
Susan, L
机构
[1] Penn State Univ, Dept Geosci, University Pk, PA 16802 USA
[2] Penn State Univ, Dept Mat Sci & Engn, University Pk, PA 16802 USA
[3] Penn State Univ, Dept Energy & Geoenvironm Engn, University Pk, PA 16802 USA
基金
美国国家科学基金会;
关键词
D O I
10.1016/S0009-2541(00)00202-3
中图分类号
P3 [地球物理学]; P59 [地球化学];
学科分类号
0708 ; 070902 ;
摘要
Differences in pH between silicate-biofilm interfaces and bulk medium (Delta pH = PHinterface - pH(bulk)) were detectable with commercial microelectrodes in cultures grown in unbuffered medium (\Delta pH\ = 0.27-1.08) for an arthrobacter species, but were generally beneath detection (Delta pH < 0.04) for a streptomyces species. Biofilm half-thicknesses developed by Arthrobacter ranged from 1.2 to 11.5 mm, and were highly variable even for replicates. In buffered medium, neither bacterium produced a measurable <Delta>pH across the biofilms grown on silicates. The silicates consisted of polished hornblende, synthetic Fe-rich glass similar to hornblende in bulk composition, and two commercially available "float glasses," one low-Fe and one high-Fe. The two species of soil bacteria investigated are both known to accelerate release of Fe from hornblende. For the Arthrobacter, values of \Delta pH\ developed on hornblende crystal or glass substrates were generally larger than those developed on either float glass. Differences in Delta pH developed on different substrates could not be related simply to relative rates of dissolution of substrates. Differences between the two bacterial species are probably related to differences in (1) rates of growth, (2) production of low-molecular-weight organic acids, (3) physical characteristics of polysaccharide slimes excreted and/or (4) production of siderophores. Although values of \Delta pH\ developed at mineral-water interfaces in natural systems may not be as large as those measured here except for water-saturated systems, it is probable that significant values of Delta pH (less than or equal to pH unit) develop where fast-growing, acid-producing microbes colonize slow-dissolving phases in the presence of unbuffered solutions. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:1 / 16
页数:16
相关论文
共 44 条
  • [21] Role of bacterial siderophores in dissolution of hornblende
    Liermann, LJ
    Kalinowski, BE
    Brantley, SL
    Ferry, JG
    [J]. GEOCHIMICA ET COSMOCHIMICA ACTA, 2000, 64 (04) : 587 - 602
  • [22] MICROBIOLOGICALLY INFLUENCED CORROSION OF METALS AND ALLOYS
    LITTLE, B
    WAGNER, P
    MANSFELD, F
    [J]. INTERNATIONAL MATERIALS REVIEWS, 1991, 36 (06) : 253 - 272
  • [23] Little BJ, 1997, REV MINERAL, V35, P123
  • [24] MALINOSKAYA I M, 1990, Mikrobiologiya, V59, P70
  • [25] MANIATIS T, 1982, MOL CLONING LABORATO, P440
  • [26] MEDLIN J. H., 1969, Fertilizer Abstracts, V2
  • [27] The influence of natural mineral coatings on feldspar weathering
    Nugent, MA
    Brantley, SL
    Pantano, CG
    Maurice, PA
    [J]. NATURE, 1998, 395 (6702) : 588 - 591
  • [28] BIOMASS AND ORGANIC-ACIDS IN SANDSTONE OF A WEATHERING BUILDING - PRODUCTION BY BACTERIAL AND FUNGAL ISOLATES
    PALMER, RJ
    SIEBERT, J
    HIRSCH, P
    [J]. MICROBIAL ECOLOGY, 1991, 21 (03) : 253 - 266
  • [29] REVSBECH NP, 1986, ADV MICROB ECOL, V9, P293
  • [30] MICROELECTRODE STUDIES OF INTERSTITIAL WATER CHEMISTRY AND PHOTOSYNTHETIC ACTIVITY IN A HOT-SPRING MICROBIAL MAT
    REVSBECH, NP
    WARD, DM
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 48 (02) : 270 - 275