Use of electron microscopic and immunogold labeling techniques to determine polyomavirus recombinant VP1 capsid-like particles entry into mouse 3T6 cell nucleus

被引：11

作者：

An, K ^{[1
]}

Paulsen, AQ ^{[1
]}

Tilley, MB ^{[1
]}

Consigli, RA ^{[1
]}

机构：

[1] Kansas State Univ, Sect Virol & Oncol, Div Biol, Manhattan, KS 66506 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 2000年 / 90卷 / 01期

基金：

美国国家科学基金会; 美国国家航空航天局;

关键词：

polyomavirus; recombinant capsid-like particles; electron microscopy; immunogold labeling; cell entry;

D O I：

10.1016/S0166-0934(00)00219-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Murine polyomavirus major structural protein VP1 could assemble into capsid-like particles when expressed in the baculovirus system. The recombinant capsid-like particles that were purified by CsCl density gradient centrifugation were capable of packaging host DNA. Electron microscopic and immunogold labeling techniques were used to study the entry of these VP1 recombinant capsid-like particles into mouse 3T6 cells. It was found that these VP1 recombinant capsid-like particles, which lack polyomavirus minor structural proteins (VP2 and VP3), use the same mechanism to enter mouse 3T6 cell cytoplasm and nucleus as that used by native polyomavirus virions. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：91 / 97

页数：7

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