A Type VI Secretion System of Pseudomonas aeruginosa Targets, a Toxin to Bacteria

被引：693

作者：

Hood, Rachel D. ^{[1
]}

Singh, Pragya ^{[2
]}

Hsu, FoSheng ^{[1
]}

Guevener, Tuezuen ^{[1
]}

Carl, Mike A. ^{[1
]}

Trinidad, Rex R. S. ^{[1
]}

Silverman, Julie M. ^{[1
]}

Ohlson, Brooks B. ^{[1
]}

Hicks, Kevin G. ^{[1
]}

Plemel, Rachael L. ^{[3
]}

Li, Mo ^{[1
]}

Schwarz, Sandra ^{[1
]}

Wang, Wenzhuo Y. ^{[1
]}

Merz, Alexey J. ^{[3
]}

Goodlett, David R. ^{[2
]}

Mougous, Joseph D. ^{[1
]}

机构：

[1] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA

[2] Univ Washington, Dept Med Chem, Seattle, WA 98195 USA

[3] Univ Washington, Dept Biochem, Seattle, WA 98195 USA

来源：

CELL HOST & MICROBE | 2010年 / 7卷 / 01期

基金：

美国国家卫生研究院;

关键词：

SMALL-COLONY VARIANTS; CYSTIC-FIBROSIS; PROTEIN SECRETION; III SECRETION; IN-VIVO; GENETIC-DETERMINANTS; BIOFILM DEVELOPMENT; ESCHERICHIA-COLI; VIBRIO-CHOLERAE; STRESS-RESPONSE;

D O I：

10.1016/j.chom.2009.12.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The functional spectrum of a secretion system is defined by its substrates. Here we analyzed the secretomes of Pseudomonas aeruginosa mutants altered in regulation of the Hcp Secretion Island-I-encoded type VI secretion system (H1-T6SS). We identified three substrates of this system, proteins Tse1-3 (type six exported 1-3), which are coregulated with the secretory apparatus and secreted under tight posttranslational control. The Tse2 protein was found to be the toxin component of a toxin-immunity system and to arrest the growth of prokaryotic and eukaryotic cells when expressed intracellularly. In contrast, secreted Tse2 had no effect on eukaryotic cells; however, it provided a major growth advantage for P. aeruginosa strains, relative to those lacking immunity, in a manner dependent on cell contact and the H1-T6SS. This demonstration that the T6SS targets a toxin to bacteria helps reconcile the structural and evolutionary relationship between the T6SS and the bacteriophage tail and spike.

引用

页码：25 / 37

页数：13

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