A Type VI Secretion System of Pseudomonas aeruginosa Targets, a Toxin to Bacteria

被引:693
作者
Hood, Rachel D. [1 ]
Singh, Pragya [2 ]
Hsu, FoSheng [1 ]
Guevener, Tuezuen [1 ]
Carl, Mike A. [1 ]
Trinidad, Rex R. S. [1 ]
Silverman, Julie M. [1 ]
Ohlson, Brooks B. [1 ]
Hicks, Kevin G. [1 ]
Plemel, Rachael L. [3 ]
Li, Mo [1 ]
Schwarz, Sandra [1 ]
Wang, Wenzhuo Y. [1 ]
Merz, Alexey J. [3 ]
Goodlett, David R. [2 ]
Mougous, Joseph D. [1 ]
机构
[1] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA
[2] Univ Washington, Dept Med Chem, Seattle, WA 98195 USA
[3] Univ Washington, Dept Biochem, Seattle, WA 98195 USA
基金
美国国家卫生研究院;
关键词
SMALL-COLONY VARIANTS; CYSTIC-FIBROSIS; PROTEIN SECRETION; III SECRETION; IN-VIVO; GENETIC-DETERMINANTS; BIOFILM DEVELOPMENT; ESCHERICHIA-COLI; VIBRIO-CHOLERAE; STRESS-RESPONSE;
D O I
10.1016/j.chom.2009.12.007
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The functional spectrum of a secretion system is defined by its substrates. Here we analyzed the secretomes of Pseudomonas aeruginosa mutants altered in regulation of the Hcp Secretion Island-I-encoded type VI secretion system (H1-T6SS). We identified three substrates of this system, proteins Tse1-3 (type six exported 1-3), which are coregulated with the secretory apparatus and secreted under tight posttranslational control. The Tse2 protein was found to be the toxin component of a toxin-immunity system and to arrest the growth of prokaryotic and eukaryotic cells when expressed intracellularly. In contrast, secreted Tse2 had no effect on eukaryotic cells; however, it provided a major growth advantage for P. aeruginosa strains, relative to those lacking immunity, in a manner dependent on cell contact and the H1-T6SS. This demonstration that the T6SS targets a toxin to bacteria helps reconcile the structural and evolutionary relationship between the T6SS and the bacteriophage tail and spike.
引用
收藏
页码:25 / 37
页数:13
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