S-RNase uptake by compatible pollen tubes in gametophytic self-incompatibility

被引:199
作者
Luu, DT [1 ]
Qin, XK [1 ]
Morse, D [1 ]
Cappadocia, M [1 ]
机构
[1] Univ Montreal, Dept Biol, Montreal, PQ H1X 2B2, Canada
关键词
D O I
10.1038/35036623
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Many flowering plants avoid inbreeding through a genetic mechanism termed self-incompatibility. An extremely polymorphic S-locus(1) controls the gametophytic self-incompatibility system that causes pollen rejection (that is, active arrest of pollen tube growth inside the style) when an S-allele carried by haploid pollen matches one of the S-alleles present in the diploid style. The only known product of the S-locus is an S-RNase expressed in the mature style(2). The pollen component to this cell-cell recognition system is unknown and current models(3,4) propose that it either acts as a gatekeeper allowing only its cognate S-RNase to enter the pollen tube, or as an inhibitor of non-cognate S-RNases. In the latter case, all S-RNases are presumed to enter pollen tubes; thus, the two models make diametrically opposed predictions concerning the entry of S-RNases into compatible pollen. Here we use immunocytochemical labelling of pollen tubes growing in styles to show accumulation of an S-RNase in the cytoplasm of all pollen-tube haplotypes, thus providing experimental support for the inhibitor model.
引用
收藏
页码:649 / 651
页数:3
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