Surface-enhanced Raman scattering: A structure specific detection method for capillary electrophoresis

被引:64
作者
He, L [1 ]
Natan, MJ [1 ]
Keating, CD [1 ]
机构
[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA
关键词
D O I
10.1021/ac000583v
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new approach to detecting capillary electrophoresis (CE) eluent components by interfacing CE with a surface-enhanced Raman scattering (SERS) system is described. In this approach, CE-based separation of a mixture of trans-1,2-bis(4-pyridyl)ethylene and N,N-dimethyl-4-nitrosoaniline has been detected by SERS in a postcolumn Geometry. The retention time obtained from SERS corresponds well with that from conventional UV-visible detection. Meanwhile, CE eluants are identified by their characteristic vibrational spectra, demonstrating the validity of SERS as a structure-specific detection method for CE. In addition, the ability to monitor SERS intensity changes at molecule-specific frequencies makes selective detection of individual analytes possible, even when separation is incomplete. Finally, CE-SERS is evaluated for separation of amino acids (tyrosine and tryptophan) and environmental pollutants (chlorophenol mixtures).
引用
收藏
页码:5348 / 5355
页数:8
相关论文
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