TcRho1 of Trypanosoma cruzi:: role in metacyclogenesis and cellular localization

被引：13

作者：

de Melo, LDB

Nepomuceno-Silva, JL

Sant'Anna, C

Eisele, N

Ferraro, RB

Meyer-Fernandes, JR

de Souza, W

Cunha-e-Silva, NL

Lopes, UG ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, CCS, Inst Biofis Carlos Chagas Filho, Mol Parasitol Lab, Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, CCS, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, Rio De Janeiro, Brazil

[3] Univ Fed Rio de Janeiro, CCS, Dept Bioquim Med, Lab Bioquim Celular, Rio De Janeiro, Brazil

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 323卷 / 03期

关键词：

Trypanosoma cruzi; GTPase; Rho; TcRho1;

D O I：

10.1016/j.bbrc.2004.08.197

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we have investigated the function of TcRho1, a Rho family orthologue from the parasite Trypanosoma cruzi. We have selected parasites overexpressing wild-type TeRho1 and a truncated form of TeRho1 (TcRho1-DeltaCaaX) which is unable to undergo farnesylation and supposed to interfere with recruitment of Rho effectors to membranes. TcRho1 protein was localized at the anterior region of wild-type and TcRho1 overexpressing epimastigotes, suggesting association with the Golgi apparatus. Accordingly, parasites overexpressing TcRho1-DeltaCaaX presented cytoplasmic fluorescence. To address the function of TcRho1 during differentiation, from epimastigotes to trypomastigotes, we submitted parasites overexpressing the above-cited lineages to metacyclogenesis assays. Parasites overexpressing TcRho1-ACaaX generated a discrete number of metacyclic trypomastigotes when Compared with other lineages. strikingly, TcRho1-DeltaCaaX cells died synchronously during the process of metacyclogenesis. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：1009 / 1016

页数：8

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[11] GTPases in protozoan parasites: Tools for cell biology and chemotherapy [J].