Modulation of glutamate sensitivities by inhibitors of a protein kinase and a protein phosphatase in cultured rat Purkinje cells

We examined the effects of inhibitors of calcium-calmodulin-dependent protein kinase II (CaM kinase II) and protein phosphatases on the glutamate (Glu) responses in cultured rat cerebellar Purkinje cells. CaM kinase II inhibitors significantly potentiated Glu responses, and activation of metabotropic Glu receptors facilitated this potentiation. In contrast, a phosphatase inhibitor calyculin A significantly reduced Glu responses. It was suggested that the Glu responsiveness of Purkinje cells may be regulated by the dynamic balance of phosphorylation and dephosphorylation of receptors or other relevant factors under basal conditions. (C) 1998 Elsevier Science Ireland Ltd.