Novel photoreactive cinnamic acid analogues to elucidate phenylalanine ammonia-lyase

被引：15

作者：

Hashimoto, M

Hatanaka, Y

Nabeta, K

机构：

[1] Obihiro Univ Agr & Vet Med, Dept Bioresource Sci, Obihiro, Hokkaido 0808555, Japan

[2] Toyama Med & Pharmaceut Univ, Inst Nat Med, Toyama 9300194, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2000年 / 10卷 / 21期

关键词：

D O I：

10.1016/S0960-894X(00)00499-6

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

4-[3-(Trifluoromethyl) diazirinyl] cinnamic acid derivatives were synthesized to elucidate properties of phenylalanine ammonia-lyase (PAL). 2-Methoxy and 2-biotinylated alkoxy compounds have inhibitory activity on the formation of phenylalanine from cinnamic acid. Specific photolabeling of the enzyme was detected using biotinylated derivatives without the use of radioisotopes. The results indicated that the 4-[3-(trifluoromethyl) diazirinyl] skeleton will be a suitable photoreactive compound to elucidate regulation of phenylpropanoid biosynthesis. (C) 2000 Elsevier Science Ltd. All rights reserved.

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页码：2481 / 2483

页数：3

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