Design of high-throughput methods of protein production for structural biology

被引:171
作者
Stevens, RC
机构
[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
[2] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
关键词
D O I
10.1016/S0969-2126(00)00193-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
引用
收藏
页码:R177 / R185
页数:9
相关论文
共 92 条
[51]   A THIOREDOXIN GENE FUSION EXPRESSION SYSTEM THAT CIRCUMVENTS INCLUSION BODY FORMATION IN THE ESCHERICHIA-COLI CYTOPLASM [J].
LAVALLIE, ER ;
DIBLASIO, EA ;
KOVACIC, S ;
GRANT, KL ;
SCHENDEL, PF ;
MCCOY, JM .
BIO-TECHNOLOGY, 1993, 11 (02) :187-193
[52]  
LAVALLIE ER, 1994, CURRENT PROTOCOLS MO
[53]  
LEE SC, 1992, J BIOL CHEM, V267, P2849
[54]   Microdrop screening: A rapid method to optimize solvent conditions for NMR spectroscopy of proteins [J].
Lepre, CA ;
Moore, JM .
JOURNAL OF BIOMOLECULAR NMR, 1998, 12 (04) :493-499
[55]   Advances in refolding of proteins produced in E-coli [J].
Lilie, H ;
Schwarz, E ;
Rudolph, R .
CURRENT OPINION IN BIOTECHNOLOGY, 1998, 9 (05) :497-501
[56]   The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes [J].
Liu, QH ;
Li, MZ ;
Leibham, D ;
Cortez, D ;
Elledge, SJ .
CURRENT BIOLOGY, 1998, 8 (24) :1300-1309
[57]  
LORBER B, 1992, CRYSTALLIZATION NUCL, P19
[58]  
Lu ZJ, 1996, J BIOL CHEM, V271, P5059
[59]   Strategies for achieving high-level expression of genes in Escherichia coli [J].
Makrides, SC .
MICROBIOLOGICAL REVIEWS, 1996, 60 (03) :512-+
[60]  
MARSTON FAO, 1990, METHOD ENZYMOL, V182, P264