Specific monoclonal antibody raised against the p33ING1 tumor suppressor

被引：16

作者：

Garkavtsev, I

Boland, D

Mai, J

Wilson, H

Veillette, C

Riabowol, K ^{[1
]}

机构：

[1] Univ Calgary, Dept Med Chem, Calgary, AB T2N 4N1, Canada

[2] Univ Calgary, So Alberta Canc Res Ctr, Calgary, AB T2N 4N1, Canada

[3] SACRC hybridoma Facil, Calgary, AB T2N 4N1, Canada

来源：

HYBRIDOMA | 1997年 / 16卷 / 06期

关键词：

D O I：

10.1089/hyb.1997.16.537

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An IgG(1) mouse monoclonal antibody (CAb1) was produced against human recombinant p33(ING1). The antibody is able to recognize native and denatured antigen in ELISA and Western blot protocols, respectively. CAb1 can be used to specifically detect the p33(ING1) protein in dot blot and Western immunoblot protocols of both human and mouse cell lysates. In addition, this antibody is also useful for cellular localization of native and ectopically overexpressed p33(ING1) protein by indirect immunofluorescence.

引用

页码：537 / 540

页数：4

共 10 条

[1] Extension of the replicative life span of human diploid fibroblasts by inhibition of the p33(ING1) candidate tumor suppressor [J].