Effects of VDAC isoforms on CuZn-superoxide dismutase activity in the intermembrane space of Saccharomyces cerevisiae mitochondria

被引：16

作者：

Budzinska, Malgorzata ^{[1
]}

Galganska, Hanna ^{[1
]}

Wojtkowska, Malgorzata ^{[1
]}

Stobienia, Olgierd ^{[1
]}

Kmita, Hanna ^{[1
]}

机构：

[1] Adam Mickiewicz Univ Poznan, Inst Mol Biol & Biotechnol, Lab Bioenerget, PL-61614 Poznan, Poland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 357卷 / 04期

关键词：

Saccharomyces cerevisiae; mitochondria; VDAC; CuZnSOD; redox state; Tom40; Tob55 (Sam50);

D O I：

10.1016/j.bbrc.2007.04.090

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Copper and zinc containing superoxide dismutase (CuZnSOD) is located primarily in the cytosol but a small amount of the enzyme has also been identified in the intermembrane space of mitochondria (termed here IMS CuZnSOD). Using Saccharomyces cerevisiae mutants depleted of either isoform of VDAC (voltage-dependent anion-selective channel), we have shown that the activity of IMS CuZnSOD coincides with the presence of a given VDAC isoform and changes in a growth phase dependent way. Moreover, the IMS CuZnSOD activity correlates with the levels of O-2(.-) release from mitochondria and the cytosol redox state. The latter in turn seems to influence the levels of the mitochondrial outer membrane channel protein other than VDAC. Thus, we conclude that in the case of S. cerevisiae both VDAC isoforms influence the IMS CuZnSOD activity and subsequently the expression levels of some mitochondrial proteins. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：1065 / 1070

页数：6

共 34 条

[31] The voltage-dependent anion channel (VDAC): Function in intracellular signalling, cell life and cell death [J].