The role of specimen-induced spherical aberration in confocal microscopy

被引：57

作者：

Török, P

Hewlett, SJ

Varga, P

机构：

[1] Univ Cambridge, Multiimaging Ctr, Cambridge CB2 3DY, England

[2] Univ Dublin Trinity Coll, Dept Phys, Dublin 2, Ireland

[3] Res Inst Mat Sci, H-1525 Budapest, Hungary

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1997年 / 188卷

关键词：

confocal brightfield microscope; confocal fluorescence microscope; coverslip thickness; point spread function; spherical aberration; stratified medium;

D O I：

10.1046/j.1365-2818.1997.2440802.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We present an overview of recent theories for describing specimen-induced spherical aberration in confocal microscopy. One of these theories is used to compute numerically the role of spherical aberration in general confocal, and especially in biological confocal, microscopy for a variety of three-layer specimen structures. In particular, we study the effect of specimen-induced spherical aberration on the maximum value of the overall confocal point spread function, the accompanying focal shift and the size of the optical probe in both fluorescence and brightfield confocal microscopy.

引用

页码：158 / 172

页数：15

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