Mechanism of genetic exchange in American trypanosomes

被引:279
作者
Gaunt, MW
Yeo, M
Frame, IA
Stothard, JR
Carrasco, HJ
Taylor, MC
Mena, SS
Veazey, P
Miles, GAJ
Acosta, N
de Arias, AR
Miles, MA
机构
[1] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Dis, London WC1E 7HT, England
[2] Nat Hist Museum, Dept Zool, London SW7 5BD, England
[3] Cent Univ Venezuela, Fac Med, Caracas, Venezuela
[4] Univ Nacl Asuncion, Inst Invest Ciencias Salud, Dept Trop Med, Asuncion, Paraguay
基金
英国惠康基金; 加拿大自然科学与工程研究理事会;
关键词
D O I
10.1038/nature01438
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The kinetoplastid Protozoa are responsible for devastating diseases(1). In the Americas, Trypanosoma cruzi is the agent of Chagas' disease-a widespread disease transmissible from animals to humans (zoonosis)-which is transmitted by exposure to infected faeces of blood-sucking triatomine bugs(2). The presence of genetic exchange in T. cruzi and in Leishmania is much debated(3,4). Here, by producing hybrid clones, we show that T. cruzi has an extant capacity for genetic exchange. The mechanism is unusual and distinct from that proposed for the African trypanosome, Trypanosoma brucei(5). Two biological clones(6) of T. cruzi were transfected to carry different drug-resistance markers(7,8), and were passaged together through the entire life cycle. Six double-drug-resistant progeny clones, recovered from the mammalian stage of the life cycle, show fusion of parental genotypes, loss of alleles, homologous recombination, and uniparental inheritance of kinetoplast maxicircle DNA. There are strong genetic parallels between these experimental hybrids and the genotypes among natural isolates of T. cruzi. In this instance, aneuploidy through nuclear hybridization results in recombination across far greater genetic distances than mendelian genetic exchange. This mechanism also parallels genome duplication(9,10).
引用
收藏
页码:936 / 939
页数:5
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