RNase E is involved in 5′-end 23S rRNA processing in α-Proteobacteria

被引：8

作者：

Klein, F ^{[1
]}

Evguenieva-Hackenberg, E ^{[1
]}

机构：

[1] Univ Giessen, Inst Mikrobiol & Mol Biol, D-35392 Giessen, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 299卷 / 05期

关键词：

alpha-Proteobacteria; internal transcribed spacer; intervening sequence; Rhodobacter capsulatus; Rhizobium leguminosarum; rRNA processing; RNA decay; RNase E; RNase III; 23S rRNA;

D O I：

10.1016/S0006-291X(02)02738-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Rhodobacter capsulatus and Rhizobium leguminosarum, an internal transcribed spacer consisting of helices 9 and 10 is removed during 23S rRNA processing, which leads to the occurrence of a 5.8S-like rRNA. The particular rRNA maturation steps are not known, with exception of the initial RNase III cleavage in helix 9. We found that GC-rich stem-loop structures of helix 9, which are released by RNase III, are immediately degraded. The degradation of helix 10 is slower and its kinetics differs in both species. Nevertheless, the helix 10 processing mechanism is conserved and includes cleavages by RNase E. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：780 / 786

页数：7

共 33 条

[21] Ribonuclease E is a 5′-end-dependent endonuclease [J].

Mackie, GA .

NATURE, 1998, 395 (6703) :720-723

[22] Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure [J].

Mathews, DH ;

Sabina, J ;

Zuker, M ;

Turner, DH .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 288 (05) :911-940

[23]

MILLIGAN JF, 1989, METHOD ENZYMOL, V180, P51

[24]

MISRA TK, 1979, J BIOL CHEM, V254, P1154

[25] DECAY OF THE IS10 ANTISENSE RNA BY 3' EXORIBONUCLEASES - EVIDENCE THAT RNASE-II STABILIZES RNA-OUT AGAINST PNPASE ATTACK [J].

PEPE, CM ;

MASLESAGALIC, S ;

SIMONS, RW .

MOLECULAR MICROBIOLOGY, 1994, 13 (06) :1133-1142

[26] INTERVENING SEQUENCE WITH CONSERVED OPEN READING FRAME IN EUBACTERIAL 23S RIBOSOMAL-RNA GENES [J].

RALPH, D ;

MCCLELLAND, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (14) :6864-6868

[27]

Rauhut R, 1999, FEMS MICROBIOL REV, V23, P353, DOI 10.1111/j.1574-6976.1999.tb00404.x

[28] Sequences around the fragmentation sites of the large subunit ribosomal RNA in the family Rhizobiaceae [J].

Selenska-Pobell, S ;

Döring, H .

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 1998, 73 (01) :55-67

[29] FRAGMENTATIONS OF THE LARGE-SUBUNIT RIBOSOMAL-RNA IN THE FAMILY RHIZOBIACEAE [J].

SELENSKAPOBELL, S ;

EVGUENIEVAHACKENBERG, E .

JOURNAL OF BACTERIOLOGY, 1995, 177 (23) :6993-6998

[30]

VONGABAIN A, 1983, P NATL ACAD SCI-BIOL, V80, P653

← 1 2 3 4 →