ClC-5 regulates dentin development through TGF-β1 pathway

被引：18

作者：

Duan, Xiaohong ^{[1
]}

Mao, Yong ^{[2
]}

Yang, Ting ^{[1
]}

Wen, Xuan ^{[1
]}

Wang, Huan ^{[3
]}

Hou, Jin ^{[1
]}

Xue, Yang ^{[4
]}

Zhang, Rong ^{[5
]}

机构：

[1] Fourth Mil Med Univ, Sch Stomatol, Dept Oral Biol, Xian 710032, Shaanxi, Peoples R China

[2] Fourth Mil Med Univ, Sch Stomatol, Dept Prosthodont, Xian 710032, Shaanxi, Peoples R China

[3] Fourth Mil Med Univ, Sch Stomatol, Dept Orthodont, Xian 710032, Shaanxi, Peoples R China

[4] Fourth Mil Med Univ, Sch Stomatol, Dept Oral Surg, Xian 710032, Shaanxi, Peoples R China

[5] Fourth Mil Med Univ, Sch Stomatol, Dept Endodont, Xian 710032, Shaanxi, Peoples R China

来源：

ARCHIVES OF ORAL BIOLOGY | 2009年 / 54卷 / 12期

基金：

中国国家自然科学基金;

关键词：

ClC-5; Dentin; Odontoblast; TGF-beta; 1; Chloride channel; CHLORIDE CHANNELS; DENTINOGENESIS IMPERFECTA; TISSUE DISTRIBUTION; MOUSE MODEL; EXPRESSION; CELLS; LOCALIZATION; DISEASES; CLONING; KIDNEY;

D O I：

10.1016/j.archoralbio.2009.09.008

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

ClC-5 is one of the voltage-dependent chloride channel (ClC) family members. Mutations involving CLCN5 cause an X-linked nephropathy associated with Dent's disease. Some Clcn5 gene knockout (ClC-5 KO) mice have abnormal growth of the teeth; however, the expression and function of ClC-5 during tooth development is still unknown. Herein we report abnormal dentin structure, decreased DSPP and increased TGF-beta 1 protein level in ClC-5 KO teeth. In odontoblast-like MDPC-23 cells, the mRNA levels of Tgfb1, Dspp and Dmp-1 were upregulated with Clcn5 RNAi after 48 h treatment; whilst there was no change in those of TGF-beta receptor Tgfbr1 and Tgfbr2. We suggest that the dentin changes in ClC-5 KO mice might be a result of increasing TGF-beta 1, and the interplay between ClC-5 and TGF-beta 1 needs further identified. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1118 / 1124

页数：7

共 24 条

[1] High citrate diet delays progression of renal insufficiency in the ClC-5 knockout mouse model of Dent's disease [J].

Cebotaru, V ;

Kaul, S ;

Devuyst, O ;

Cai, H ;

Racusen, L ;

Guggino, WB ;

Guggino, SE .

KIDNEY INTERNATIONAL, 2005, 68 (02) :642-652

[2] Involvement of chloride channels in TGF-β1-induced apoptosis of human bronchial epithelial cells [J].

Cheng, Gang ;

Shao, Zhifei ;

Chaudhari, Bharti ;

Agrawal, Devendra K. .

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2007, 293 (05) :L1339-L1347

[3] Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig C1C-5 chloride channel [J].

Cornejo, I ;

Niemeyer, MI ;

Sepúlveda, FV ;

Cid, LP .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2001, 1512 (02) :367-374

[4]

Duan X, 2004, J DENT RES, V83, P2741

[5] Pre- and postnatal lung development, maturation, and plasticity - ClC-5: ontogeny of an alternative chloride channel in respiratory epithelia [J].

Edmonds, RD ;

Silva, IV ;

Guggino, WB ;

Butler, RB ;

Zeitlin, PL ;

Blaisdell, CJ .

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2002, 282 (03) :L501-L507

[6] Overexpression of transforming growth factor-β1 in teeth results in detachment of ameloblasts and enamel defects [J].

Haruyama, Naoto ;

Thyagarajan, Tamizchelvi ;

Skobe, Ziedonis ;

Wright, J. Tim ;

Septier, Dominique ;

Sreenath, Taduru L. ;

Goldberg, Michel ;

Kulkarni, Ashok B. .

EUROPEAN JOURNAL OF ORAL SCIENCES, 2006, 114 :30-34

[7]

HOU J, 2007, HUA XI KOU QIANG YI, V25, P453

[8] ClC chloride channels in tooth germ and odontoblast-like MDPC-23 cells [J].

Hou, Jin ;

Situ, Zhenqiang ;

Duan, Xiaohong .

ARCHIVES OF ORAL BIOLOGY, 2008, 53 (09) :874-878

[9]

Hou Jin, 2007, Hua Xi Kou Qiang Yi Xue Za Zhi, V25, P444

[10] Molecular structure and physiological function of chloride channels [J].

Jentsch, TJ ;

Stein, V ;

Weinreich, F ;

Zdebik, AA .

PHYSIOLOGICAL REVIEWS, 2002, 82 (02) :503-568

← 1 2 3 →