Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig C1C-5 chloride channel

被引：8

作者：

Cornejo, I

Niemeyer, MI

Sepúlveda, FV

Cid, LP

机构：

[1] Ctr Estudios Cient, Valdivia, Chile

[2] Univ Chile, Fac Med, ICBM, Santiago 7, Chile

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2001年 / 1512卷 / 02期

关键词：

small intestine; epithelial transport; chloride channel; guinea pig chloride channel protein-5; patch clamp;

D O I：

10.1016/S0005-2736(01)00331-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the cloning of a guinea pig ClC-5 chloride channel (gpClC-5) from distal small intestinal epithelial cells by RT-PCR and RACE. The transcript is shown to be present in duodenum, jejunum and ileum epithelium by RT-PCR and Northern analysis. This is confirmed by in situ hybridisation which also shows the transcript to be homogeneously distributed in the crypt and villus regions. Expression of gpClC-5 in HEK-293 cells generated markedly outwardly rectified chloride currents with a perm-selectivity sequence of NO3- > Cl- > Br- > I- > F- > gluconate(-). The possible role of gpClC5 in this epithelial location is discussed. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：367 / 374

页数：8

共 25 条

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