CLONING AND EXPRESSION OF A PROTEIN-KINASE C-REGULATED CHLORIDE CHANNEL ABUNDANTLY EXPRESSED IN RAT-BRAIN NEURONAL CELLS

被引：219

作者：

KAWASAKI, M

UCHIDA, S

MONKAWA, T

MIYAWAKI, A

MIKOSHIBA, K

MARUMO, F

SASAKI, S

机构：

[1] UNIV TOKYO,INST MED SCI,DEPT MOLEC NEUROL,MINATO KU,TOKYO 108,JAPAN

[2] KEIO UNIV,SCH MED,DEPT INTERNAL MED,SHINJUKU KU,TOKYO 160,JAPAN

来源：

NEURON | 1994年 / 12卷 / 03期

关键词：

D O I：

10.1016/0896-6273(94)90215-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

cDNA (CIC-3) encoding a protein kinase C-regulated chloride channel was cloned and characterized. The open reading frame encodes 760 amino acids, which possess significant amino acid identity with previously cloned CIC chloride channels. The chloride currents expressed in Xenopus oocytes injected with CIC-3 cRNA were completely blocked by activation of protein kinase C by 12-O-tetradecanoylphorbol 13-acetate. Abundant expression of CIC-3, mRNA was observed in rat brain, especially in the olfactory bulb, hippocampus, and cerebellum. These findings suggest that CIC-3 may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C.

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页码：597 / 604

页数：8

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