Fluorescence turn-on detection of protamine based on aggregation-induced emission enhancement characteristics of 4-(6′-carboxyl)hexyloxysalicylaldehyde azine

被引：61

作者：

Chen, Xiao-tong ^{[1
]}

Xiang, Yu ^{[1
]}

Li, Na ^{[1
]}

Song, Pan-Shu ^{[1
]}

Tong, Ai-jun ^{[1
]}

机构：

[1] Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China

来源：

ANALYST | 2010年 / 135卷 / 05期

基金：

中国国家自然科学基金;

关键词：

INTRAMOLECULAR PROTON-TRANSFER; ASSAY; HEPARIN; FLUOROPHORES; DNA; ACETYLCHOLINESTERASE; DERIVATIVES; ELECTRODES; CONVENIENT; SENSORS;

D O I：

10.1039/b925508g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel fluorescence turn-on method for sensitive and selective detection of protamine was developed based on aggregation-induced emission enhancement (AIEE) characteristics of 4-(6'-carboxyl)hexyloxysalicylaldehyde azine (CHSA) induced by electrostatic interaction between protamine and CHSA(-). Under optimal conditions described, a large Stokes shift of similar to 198 nm could be observed, and the fluorescence enhancement at 538 nm was linearly related to the concentration of protamine in the range of 0.6 similar to 18 mu g mL(-1) with the relative correlation coefficient of R-2 = 0.9996 (n = 11) and a detection limit as low as 43 ng mL(-1). The relative standard deviation (R. S. D.) was 2.0% (n = 5). The proposed method was successfully utilized in quantifying protamine in diluted horse serum. In addition, due to the special electrostatic association between protamine and heparin, CHSA could also be employed as a probe to study the interaction between them.

引用

页码：1098 / 1105

页数：8

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