Variable leucine-rich repeats of tomato disease resistance genes Cf-2 and Cf-5 determine specificity

被引：19

作者：

Seear, PJ ^{[1
]}

Dixon, MS ^{[1
]}

机构：

[1] Univ Southampton, Sch Biol Sci, Southampton SO16 7PX, Hants, England

来源：

MOLECULAR PLANT PATHOLOGY | 2003年 / 4卷 / 03期

关键词：

CLADOSPORIUM-FULVUM; PROTEINS; IDENTIFICATION; BINDING; PLANTS; LOCI; AVR9;

D O I：

10.1046/j.1364-3703.2003.00162.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The tomato Cf-2 and Cf-5 genes confer race specific resistance to infection by the leaf mould pathogen Cladosporium fulvum. The encoded proteins induce a defence response upon recognition of the fungal Avr2 and Avr5 determinants, respectively. Each resistance protein is comprised largely of leucine-rich repeats (LRRs) and the specificity of recognition is thought to occur through a particular domain. We have investigated this further using domain swaps between Cf-2 and Cf-5. Engineered chimeric genes containing portions of Cf-2 and Cf 5 were expressed and shown to be functional. The results clearly show that the specificity for the particular avirulence determinant is restricted to a region of each gene that encodes a subset of LRRs containing the highest level of intergenic variability. In addition, two non-functional mutants of Cf-5 were characterized and their significance discussed.

引用

页码：199 / 202

页数：4

共 16 条

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