Protective effect of Enterococcus faecium J96, a potential probiotic strain, on chicks infected with Salmonella pullorum

被引:86
作者
Audisio, MC
Oliver, G
Apella, MC
机构
[1] Univ Nacl Tucuman, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
[2] Ctr Referencia Lactobacilos, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
关键词
D O I
10.4315/0362-028X-63.10.1333
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Enterococcus faecium J96 was isolated from a healthy free-range chicken and it inhibited Salmonella Pullorum, in vitro, due to its lactic acid and bacteriocin production. Ln vivo assays were carried out with 30-h-old broiler chicks. The lactic acid bacteria (similar to 1 x 10(9) cells per chick) were orally administered as preventive and as therapeutic treatments. In the first case they were given to the chicks twice a day for 3 consecutive days. In the second case the lactic bacteria were administered in the same way after a 24-h challenge by Salmonella Pullorum tin both instances the salmonella dose was 1 x 10(5) cells per chick). Cecal contents, liver, and spleens were analyzed and liver and spleen fragments were also fixed in formaldehyde (pH 7.00) in order to determine salmonella translocation. The chickens that were preventively treated with E, faecium J96 survived the Salmonella Pullorum challenge. Those that were infected on the first day and then inoculated with lactic bacteria died 4 days later. Salmonellae were isolated from their livers and spleens. From these results we may conclude that E. faecium J96 can protect newly hatched chicks from Salmonella Pullorum infection but cannot act as a good therapeutic agent.
引用
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页码:1333 / 1337
页数:5
相关论文
共 32 条
[21]   NEW ASPECTS OF SALMONELLA INFECTION IN BROILER PRODUCTION [J].
NURMI, E ;
RANTALA, M .
NATURE, 1973, 241 (5386) :210-211
[22]  
PESCE MLD, 1998, THESIS U NACL TUCUMA
[23]  
Pivnick H., 1982, Developments in Food Microbiology, V1, P41
[24]   STUDIES ON BACTERIAL FLORA OF ALIMENTARY TRACT OF PIGS .2. STREPTOCOCCI - SELECTIVE ENUMERATION AND DIFFERENTIATION OF DOMINANT GROUP [J].
RAIBAUD, P ;
MOCQUOT, G ;
CAULET, M ;
GALPIN, JV .
JOURNAL OF APPLIED BACTERIOLOGY, 1961, 24 (03) :285-&
[25]  
Rolfe RD., 1991, COLONIZATION CONTROL, P59
[26]  
Sandoval V. E., 1989, Revista Argentina de Produccion Animal, V9, P295
[27]  
Schneitz C, 1998, LETT APPL MICROBIOL, V26, P338, DOI 10.1046/j.1472-765X.1998.00343.x
[28]   THE VIRULENCE OF SALMONELLA STRAINS FOR CHICKENS - THEIR EXCRETION BY INFECTED CHICKENS [J].
SMITH, HW ;
TUCKER, JF .
JOURNAL OF HYGIENE, 1980, 84 (03) :479-488
[29]  
Snoeyenbos G. H., 1991, Diseases of poultry., P73
[30]  
TERZOLO HR, 1999, COMMUNICATION