Site-specific PEGylation of proteins containing unnatural amino acids

被引：207

作者：

Deiters, A

Cropp, TA

Summerer, D

Mukherji, M

Schultz, PG

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2004年 / 14卷 / 23期

关键词：

D O I：

10.1016/j.bmcl.2004.09.059

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Here, we report a generally applicable PEGylation methodology based on the site-specific incorporation of para-azidophenylalanine into proteins in yeast. The azido group was used in a mild [3+2] cycloaddition reaction with an alkyne derivatized PEG reagent to afford selectively PEGylated protein. This strategy should be useful for the generation of selectively PEGylated proteins for therapeutic applications. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：5743 / 5745

页数：3

共 21 条

[1] Site-specific incorporation of a redox-active amino acid into proteins
Alfonta, L
Zhang, ZW
Uryu, S
Loo, JA
Schultz, PG
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (48) : 14662 - 14663
[2] A simple technique for the simultaneous determination of molecular weight and activity of superoxide dismutase using SDS-PAGE
Chen, JR
Liao, CW
Mao, SJT
Chen, TH
Weng, CN
[J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2001, 47 (03): : 233 - 237
[3] An expanded eukaryotic genetic code
Chin, JW
Cropp, TA
Anderson, JC
Mukherji, M
Zhang, ZW
Schultz, PG
[J]. SCIENCE, 2003, 301 (5635) : 964 - 967
[4] Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
Chin, JW
Martin, AB
King, DS
Wang, L
Schultz, PG
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (17) : 11020 - 11024
[5] Addition of p-azido-L-phenylaianine to the genetic code of Escherichia coli
Chin, JW
Santoro, SW
Martin, AB
King, DS
Wang, L
Schultz, PG
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (31) : 9026 - 9027
[6] Adding amino acids with novel reactivity to the genetic code of Saccharomyces cerevisiae
Deiters, A
Cropp, TA
Mukherji, M
Chin, JW
Anderson, JC
Schultz, PG
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (39) : 11782 - 11783
[7] Mono-N-terminal poly(ethylene glycol)-protein conjugates
Kinstler, O
Molineux, G
Treuheit, M
Ladd, D
Gegg, C
[J]. ADVANCED DRUG DELIVERY REVIEWS, 2002, 54 (04) : 477 - 485
[8] A method for the generation of glycoprotein mimetics
Liu, HT
Wang, L
Brock, A
Wong, CH
Schultz, PG
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (07) : 1702 - 1703
[9] ATOMIC STRUCTURES OF WILD-TYPE AND THERMOSTABLE MUTANT RECOMBINANT HUMAN CU,ZN SUPEROXIDE-DISMUTASE
PARGE, HE
HALLEWELL, RA
TAINER, JA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (13) : 6109 - 6113
[10] Chemistry for peptide and protein PEGylation
Roberts, MJ
Bentley, MD
Harris, JM
[J]. ADVANCED DRUG DELIVERY REVIEWS, 2002, 54 (04) : 459 - 476

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