Analysis of Borrelia burgdorferi IgG antibodies with a combination of IgG ELISA and VlsE c6 peptide ELISA

被引：20

作者：

Jansson, C

Carlsson, SA

Granlund, H

Wahlberg, P

Nyman, D

机构：

[1] Aland Cent Hosp, Dept Lab, Aland, Finland

[2] Aland Borrelia Grp, Aland, Finland

[3] Aland Cent Hosp, Dept Med, Aland, Finland

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2005年 / 11卷 / 02期

关键词：

antibody detection; Borrelia burgdorferi; C6; peptide; ELISA; IgG; Lyme disease;

D O I：

10.1111/j.1469-0691.2004.01041.x

中图分类号：

R51 [传染病];

学科分类号：

100401 [流行病与卫生统计学];

摘要：

Enzyme-linked immunosorbent assays (ELISAs) were used to detect antibodies to the C6 peptide of the Borrelia burgdorferi VlsE protein and a selection of B. burgdorferi IgG antigens, separately and as a combination, in 355 serum specimens from blood donors and patients. Western immunoblotting was used as the reference method. The sensitivity of the combined analysis of IgG antigen and C6 peptide analysis was markedly superior to those of the separate analyses. When the C6 peptide and IgG results were concordant, the customary confirmatory Western immunoblotting assay could be omitted, thus reducing the time and cost of analysis.

引用

页码：147 / 150

页数：5

共 15 条

[1]

[Anonymous], QUALITATSSTANDARDS M

[2]

Serodiagnosis of Lyme disease by kinetic enzyme-linked immunosorbent assay using recombinant VlsE1 or peptide antigens of Borrelia burgdorferi compared with 2-tiered testing using whole-cell lysates [J].