Moesin is not a receptor for measles virus entry into mouse embryonic stem cells

被引:15
作者
Doi, Y
Kurita, M
Matsumoto, M
Kondo, T
Noda, T
Tsukita, S
Tsukita, S
Seya, T [1 ]
机构
[1] Osaka Med Ctr Canc & Cardiovasc Dis, Dept Immunol, Higashinari Ku, Osaka 537, Japan
[2] Kyoto Univ, Fac Med, Dept Cell Biol, Sakyo Ku, Kyoto 606, Japan
[3] Kyoto Univ, Coll Med Technol, Sakyo Ku, Kyoto 606, Japan
[4] Osaka Univ, Sch Med, Dept Internal Med 2, Osaka 565, Japan
[5] Nagoya Univ, Sch Med, Dept Internal Med 1, Showa Ku, Nagoya, Aichi 466, Japan
[6] Japanese Fdn Canc Res, Inst Canc, Dept Cell Biol, Toshima Ku, Tokyo 170, Japan
[7] PROBRAIN, Tokyo 105, Japan
关键词
D O I
10.1128/JVI.72.2.1586-1592.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The involvement of moesin in measles virus (MV) entry was investigated with moesin-positive and negative mouse embryonic stem (ES) cells. MV infection of these cells was very ineffective and was independent of moesin expression. Furthermore, when these cells were transfected to express human CD46, a 100-fold increase in syncytium formation was observed with these cells and was independent of the expression of moesin, The only obvious difference between moesin-positive and -negative ES cells was the shape of the syncytia Formed, Moesin-negative ES cells expressing or not expressing human CD46 formed sepal ate pieces of fragmented syncytia which mere torn apart during spreading, whereas ES cells expressing moesin exhibited typical syncytia. In addition, moesin was not detected on the surface of any murine cells or cell lines that we hale tested by a flow cytometric assay with moesin-specific antibodies, These findings indicate that murine moesin is neither a receptor nor a CD46 coreceptor for MV entry into mouse ES cells. Moesin is involved in actin filament-plasma membrane interactions as a cross-linker, and it affects only the spreading and shape of MV-mediated syncytia.
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收藏
页码:1586 / 1592
页数:7
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