Human NK cells display major phenotypic and functional changes over the life span

被引:230
作者
Le Garff-Tavernier, Magali [2 ,3 ]
Beziat, Vivien [2 ]
Decocq, Julie [2 ,4 ]
Siguret, Virginie [5 ,6 ]
Gandjbakhch, Frederique [2 ,7 ]
Pautas, Eric [8 ]
Debre, Patrice [2 ]
Merle-Beral, Helene [2 ,3 ,9 ]
Vieillard, Vincent [1 ,2 ]
机构
[1] Hop La Pitie Salpetriere, INSERM, Lab Immunol Cellulaire & Tissulaire, UMR S 945, F-75013 Paris, France
[2] Univ Paris 06, F-75005 Paris, France
[3] Hop La Pitie Salpetriere, AP HP, Serv Hematol, F-75013 Paris, France
[4] Lab Francais Fractionnement & Biotechnol LFB, F-91940 Les Ulis, France
[5] Hop Charles Foix, AP HP, Hematol Lab, F-94205 Ivry, France
[6] Univ Paris 05, F-75006 Paris, France
[7] Hop La Pitie Salpetriere, Serv Rhumatol, AP HP, F-75013 Paris, France
[8] Hop Charles Foix, AP HP, Serv Geriatrie, F-94205 Ivry, France
[9] INSERM, UMR S 872, Ctr Cordeliers, F-75006 Paris, France
关键词
NK cells; Cord blood; Elderly; CD94; NKG2A; Interferon gamma; cytotoxicity; NATURAL-KILLER-CELLS; ADAPTIVE IMMUNITY; INNATE IMMUNITY; CORD-BLOOD; T-CELLS; RECEPTORS; CD56(BRIGHT); IMMUNOSENESCENCE; ACTIVATION; EXPRESSION;
D O I
10.1111/j.1474-9726.2010.00584.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
P>Aging is generally associated with an increased predisposition to infectious diseases and cancers, related in part to the development of immune senescence, a process that affects all cell compartments of the immune system. Although many studies have investigated the effects of age on natural killer (NK) cells, their conclusions remain controversial because the diverse health status of study subjects resulted in discordant findings. To clarify this situation, we conducted the first extensive phenotypic and functional analysis of NK cells from healthy subjects, comparing NK cells derived from newborn (cord blood), middle-aged (18-60 years), old (60-80 years), and very old (80-100 years) subjects. We found that NK cells in cord blood displayed specific features associated with immaturity, including poor expression of KIR and LIR-1/ILT-2 and high expression of both NKG2A and IFN-gamma. NK cells from older subjects, on the other hand, preserved their major phenotypic and functional characteristics, but with their mature features accentuated. These include a profound decline of the CD56bright subset, a specific increase in LIR-1/ILT-2, and a perfect recovering of NK-cell function following IL2-activation in very old subjects. We conclude that the preservation of NK cell features until very advanced age may contribute to longevity and successful aging.
引用
收藏
页码:527 / 535
页数:9
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