Separation of proteins on polymeric stationary phases grafted with various amine groups

被引：20

作者：

Choi, SH ^{[1
]}

Hwang, YM ^{[1
]}

Lee, KP ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Dept Chem, Grad Sch, Taegu 702701, South Korea

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 987卷 / 1-2期

关键词：

stationary phases; LC; grafting; proteins; glycidyl methacrylate; ainines;

D O I：

10.1016/S0021-9673(02)01840-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Six polymeric stationary phases with various amine groups were prepared by surface grafting of glycidyl methacrylate on silica gel surface and its subsequent amination. The six kinds of amines, namely, triethylamine, diethylamine, ethylenediamine (EDA), hexaethylenediamine (HEDA), diethylenetriamine (DETA) and triethylenetetraamine (TETA) were used in this study. The separation of bovine serum albumin (BSA) and chicken egg albumin (CEA) on the polymeric stationary phase with various amines was investigated. The affinity degree of BSA was higher than CEA for the EDA, HEDA and DETA columns, whereas the affinity degree of CEA was higher than BSA for the TETA column. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：323 / 330

页数：8

共 12 条

[1] Affinity chromatography of proteins on non-porous copolymerized particles of styrene, methyl methacrylate and glycidyl methacrylate [J].